Crop Breeding

Methods and Protocols

  • Delphine Fleury
  • Ryan Whitford

Part of the Methods in Molecular Biology book series (MIMB, volume 1145)

Table of contents

  1. Front Matter
    Pages i-x
  2. Richard M. Trethowan
    Pages 1-20
  3. Elise J. Tucker, Bao Lam Huynh
    Pages 59-66
  4. Chunlin He, John Holme, Jeffrey Anthony
    Pages 75-86
  5. Sergío Perez-Elizalde, Jesús J. Cerón-Rojas, José Crossa, Delphine Fleury, Gregorio Alvarado
    Pages 87-96
  6. Fabio Valente, Franck Gauthier, Nicolas Bardol, Guylaine Blanc, Johann Joets, Alain Charcosset et al.
    Pages 97-116
  7. Mark A. Newell, Jean-Luc Jannink
    Pages 117-130
  8. Adrian Hathorn, Scott Chapman, Mark Dieters
    Pages 131-142
  9. Peter Sharp, Chongmei Dong
    Pages 155-165
  10. Sue Broughton, Parminder K. Sidhu, Philip A. Davies
    Pages 167-189
  11. Ainur Ismagul, Gulnur Iskakova, John C. Harris, Serik Eliby
    Pages 191-202
  12. Ainur Ismagul, Iryna Mazonka, Corinne Callegari, Serik Eliby
    Pages 203-211
  13. Back Matter
    Pages 253-255

About this book


The recent advent of molecular technologies has changed the way plant breeders identify and select their germplasm as genetic variation can now be assessed at the DNA level. Crop Breeding: Methods and Protocols presents detailed guidelines and tutorials that suit different needs and capacity from small laboratories analyzing molecular markers on a one-by-one basis to the increasingly popular high-throughput protocols for high capacity laboratories. Topics covered include breeding strategy for the selection of an ideal variety or genetic ideotype, protocols for breeders using molecular markers in selection programs and for laboratories providing molecular services to breeding programs, statistical programs and software to aid implementation of molecular data into breeding programs, and methodologies that facilitate the generation of genetic diversity and its characterization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Crop Breeding: Methods and Protocols will help in expanding the use of molecular technologies for the creation of tomorrow’s crop varieties.


crop improvement gene sequencing genomic selection genotyping plant breeding

Editors and affiliations

  • Delphine Fleury
    • 1
  • Ryan Whitford
    • 2
  1. 1.University of Adelaide, Australian Centre for Plant Functional Genomics (ACPFG)UrrbraeAustralia
  2. 2.University of Adelaide, Australian Centre for Plant Functional Genomics (ACPFG)UrrbraeAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0445-7
  • Online ISBN 978-1-4939-0446-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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