Herpes Simplex Virus

Methods and Protocols

  • Russell J. Diefenbach
  • Cornel Fraefel

Part of the Methods in Molecular Biology book series (MIMB, volume 1144)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Roger D. Everett
    Pages 1-17
  3. Peggy Marconi, Roberto Manservigi
    Pages 19-29
  4. William F. Goins, Shaohua Huang, Justus B. Cohen, Joseph C. Glorioso
    Pages 63-79
  5. Matias E. Melendez, Cornel Fraefel, Alberto L. Epstein
    Pages 81-98
  6. Andrea S. Laimbacher, Cornel Fraefel
    Pages 99-115
  7. Hiroshi Nakashima, E. Antonio Chiocca
    Pages 117-127
  8. Andreas Sauerbrei, Kathrin Bohn
    Pages 149-165
  9. Ju Youn Kim, Lora A. Shiflett, Jessica A. Linderman, Ian Mohr, Angus C. Wilson
    Pages 167-179
  10. Monica Miranda-Saksena, Ross Boadle, Anthony L. Cunningham
    Pages 223-234
  11. Michael Seyffert, Anna Paula de Oliveira, Cornel Fraefel, Rebecca Vogel
    Pages 235-247
  12. Ryan H. Rochat, Corey W. Hecksel, Wah Chiu
    Pages 265-281
  13. Jolene M. Draper, Graham S. Stephenson, Jennifer H. LaVail
    Pages 283-292
  14. Lorry M. Grady, Ping Bai, Sandra K. Weller
    Pages 293-304
  15. Joshua O. Marshak, Lichun Dong, David M. Koelle
    Pages 305-327
  16. Back Matter
    Pages 329-331

About this book


Herpes Simplex Virus: Methods and Protocols provides a wide collection of protocols employed in various levels of herpes virus research, including basic protocols on growing viruses in cell culture and cloning, manipulating, and preparing viral DNA. Other chapters describe approaches to design and apply HSV-1 vectors for vaccination, cancer and gene therapy, or to study specific aspects of HSV-1 biology such as latency, intracellular transport, and protein-protein interaction. Procedures for structural analyses, microscopy, proteomics, and testing of antivirals are included as well.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and authoritative, Herpes Simplex Virus: Methods and Protocols will aid new researchers in the field of herpes virology as well as those experienced investigators wishing to embark on new techniques.


Cancer and gene therapy HSV-1 HSV-2 Herpes viruses Vaccination Viral DNA preparation Virus/host interactions

Editors and affiliations

  • Russell J. Diefenbach
    • 1
  • Cornel Fraefel
    • 2
  1. 1.Centre for Virus ResearchWestmead Millennium InstituteWestmeadAustralia
  2. 2.Institute of VirologyUniversity of ZürichSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0427-3
  • Online ISBN 978-1-4939-0428-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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