Methods and Protocols

  • Radhakrishnan Padmanabhan
  • Subhash G. Vasudevan

Part of the Methods in Molecular Biology book series (MIMB, volume 1138)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Modern Quantitative Virological Methods

    1. Front Matter
      Pages 1-1
    2. Summer Zhang, Kuan Rong Chan, Hwee Cheng Tan, Eng Eong Ooi
      Pages 3-14
    3. Kah Hin Tan, Kitti Chan Wing Ki, Satoru Watanabe, Subhash G. Vasudevan, Manoj Krishnan
      Pages 99-109
  3. Reverse Genetic Systems to Study Virus Replication and Evolution

    1. Front Matter
      Pages 111-111
    2. Sofia L. Alcaraz-Estrada, Rosa del Angel, Radhakrishnan Padmanabhan
      Pages 131-150
    3. Moon Y. F. Tay, Chin Chin Lee, Subhash G. Vasudevan, Nicole J. Moreland
      Pages 161-173
    4. Pauline Poh Kim Aw, Paola Florez de Sessions, Andreas Wilm, Long Truong Hoang, Niranjan Nagarajan, October M. Sessions et al.
      Pages 175-195
  4. Modern Methods to Study Conformation of RNA Proteins and Their Molecular Interactions

    1. Front Matter
      Pages 197-197
    2. Wojciech K. Kasprzak, Bruce A. Shapiro
      Pages 199-224
    3. Joanna Sztuba-Solinska, Stuart F. J. Le Grice
      Pages 225-239
    4. Yin Hoe Yau, Susana Geifman Shochat
      Pages 271-284
    5. Nicholas J. Barrows, Sharon F. Jamison, Shelton S. Bradrick, Caroline Le Sommer, So Young Kim, James Pearson et al.
      Pages 285-299
    6. Johanna E. Fraser, Stephen M. Rawlinson, Chunxiao Wang, David A. Jans, Kylie M. Wagstaff
      Pages 301-328
  5. In Vitro Enzyme Assays for Antiviral Screening

    1. Front Matter
      Pages 329-329
    2. Mark Manzano, Janak Padia, Radhakrishnan Padmanabhan
      Pages 331-344
  6. Dengue Animal Model for Vaccines and Antivirals

    1. Front Matter
      Pages 375-375
    2. Emily Plummer, Sujan Shresta
      Pages 377-390
    3. Satoru Watanabe, Subhash G. Vasudevan
      Pages 391-400
  7. Back Matter
    Pages 401-403

About this book


Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.


RNA proteins antiviral screening dengue virus enzyme assays virology

Editors and affiliations

  • Radhakrishnan Padmanabhan
    • 1
  • Subhash G. Vasudevan
    • 2
  1. 1.Georgetown University Medical Center Dept. of Microbiology and ImmunologyWashingtonUSA
  2. 2.Duke-NUS GMS Singapore Emerging Infectious DiseasesSingaporeSingapore

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0347-4
  • Online ISBN 978-1-4939-0348-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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