Methods and Protocols

  • David J. Sharp

Part of the Methods in Molecular Biology book series (MIMB, volume 1136)

Table of contents

  1. Front Matter
    Pages i-x
  2. Imaging and Measurement of Mitotic Spindle Dynamics In Vivo

  3. Analysis and Manipulation of the Microtubule Cytoskeleton in Cells and In Vitro

    1. Front Matter
      Pages 79-79
    2. Jonathan Nye, Daniel W. Buster, Gregory C. Rogers
      Pages 81-101
    3. Justus Tegha-Dunghu, Eva M. Gusnowski, Martin Srayko
      Pages 103-116
  4. Specialized Biophysical and Microscopy Approaches for Analyzing Microtubules and Microtubule Associated Proteins

    1. Front Matter
      Pages 135-135
    2. Sebastian P. Maurer, Franck J. Fourniol, Andreas Hoenger, Thomas Surrey
      Pages 247-260
    3. Stefanie Redemann, Britta Weber, Marit Möller, Jean-Marc Verbavatz, Anthony A. Hyman, Daniel Baum et al.
      Pages 261-278
  5. Extras

    1. Front Matter
      Pages 279-279
    2. Fred Chang, Erdinc Atilgan, David Burgess, Nicolas Minc
      Pages 281-290
    3. Francesca Faggioli, Jan Vijg, Cristina Montagna
      Pages 291-305
  6. Back Matter
    Pages 307-308

About this book


Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell. The volume is divided into four parts, each focused on methods pertaining to distinct aspects of mitosis research.  Part I presents approaches for visualizing and analyzing the dynamic behaviors of the spindle apparatus, the microtubule based machine that drives chromosome segregation.  Part II focuses more generally on methods for studying and manipulating the microtubule cytoskeleton in cells and complex cell free extracts. Part III provides state of the art biophysical and high resolution microscopy approaches for assessing complex interactions between microtubules and microtubule-associated proteins in isolation as well as microtubule structure in cells. Part IV provides methods for studying the effects of cell shape on cell division, and methods for quantifying aneuploidy (aberrant chromosome number) which frequently results from mitotic defects and has been linked to human maladies ranging from birth defects to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Mitosis: Methods and Protocols seeks to provide diverse methods and  new techniques to address new or old questions related to the mechanisms of mitosis.


cell division chromosome segregation complex cell free extracts microtubule based machine microtubule cytoskeleton in cells microtubule structure in cells microtubule-associated proteins microtubules mitosis mitotic defects quantifying aneuploidy spindle apparatus

Editors and affiliations

  • David J. Sharp
    • 1
  1. 1.Dept Physiology BiophysicsAlbert Einstein College of MedicineBronxUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0328-3
  • Online ISBN 978-1-4939-0329-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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