Cerebral Angiogenesis

Methods and Protocols

  • Richard Milner

Part of the Methods in Molecular Biology book series (MIMB, volume 1135)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Overview of Cerebral Angiogenesis

    1. Front Matter
      Pages 1-1
    2. Ina M. Wittko-Schneider, Fabian T. Schneider, Karl H. Plate
      Pages 3-20
    3. Jisook Lee, Andrew Baird, Brian P. Eliceiri
      Pages 25-34
  3. Animal Models of Cerebral Angiogenesis

    1. Front Matter
      Pages 53-53
    2. Nicole Ziegler, Karl H. Plate, Stefan Liebner
      Pages 55-68
    3. Constantinos P. Tsipis, Xiaoyan Sun, Kui Xu, Joseph C. LaManna
      Pages 69-80
    4. Masafumi Ihara, Akihiko Taguchi, Takakuni Maki, Kazuo Washida, Hidekazu Tomimoto
      Pages 95-102
    5. Kristian P. Doyle, Marion S. Buckwalter
      Pages 103-110
    6. Gina Hadley, Michalis Papadakis, Alastair M. Buchan
      Pages 111-120
    7. André Duelsner, Nora Gatzke, Anja Bondke Persson, Ivo R. Buschmann
      Pages 121-125
    8. Shea Hughes, Oleksandr Dashkin, Richard Anthony DeFazio
      Pages 127-138
    9. Kiersten L. Berggren, Jacob J. M. Kay, Rodney A. Swain
      Pages 139-154
  4. Methods of Examining Cerebral Angiogenesis

    1. Front Matter
      Pages 155-155
    2. Moises Freitas-Andrade, Jacqueline Slinn, Claudie Charlebois, Maria J. Moreno
      Pages 157-175
    3. Amin Boroujerdi, Jennifer V. Welser-Alves, Richard Milner
      Pages 177-186
    4. Michel Mittelbronn, Peter Baumgarten, Patrick N. Harter, Karl H. Plate
      Pages 187-203

About this book


Providing a selection of the key techniques that are used in characterizing cerebral angiogenesis, Cerebral Angiogenesis: Methods and Protocols aims to define the cellular and molecular mechanisms underlying this important process.  Divided into six parts, this detailed volume examines cerebral angiogenesis occurring in different scenarios, a variety of different models in which cerebral angiogenesis can be studied, methods to characterize and quantify angiogenic events as well as several different approaches to measure changes in cerebral blood flow, different approaches to investigate the role of specific candidate genes in cerebral angiogenesis, methods of therapeutically manipulating cerebral angiogenesis by gene delivery, and finally important approaches to examine cerebral angiogenic mechanisms in vitro.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and decidedly practical, Cerebral Angiogenesis: Methods and Protocols should be a vital resource for all researchers, both those new to this field as well as those looking to use more specialized and sophisticated techniques to examine blood vessel growth in the brain.


Blood vessel growth Blood-brain barrier Brain blood vessels Central nervous system Cerebral blood flow Endothelial cell behavior Gene delivery

Editors and affiliations

  • Richard Milner
    • 1
  1. 1.Scripps Research InstituteLa JollaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0320-7
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0319-1
  • Online ISBN 978-1-4939-0320-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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