Enzyme Engineering

1. Enzyme Engineering

   1. Kinetics of D-Glucose to D-Fructose Isomerization

      • G. A. McKay, L. L. Tavlarides

      Pages 145-153

   2. Efficiency of Two Immobilized Enzymes Acting in Sequence

      • S. Y. Shimizu, H. M. Lenhoff

      Pages 155-161

   3. Photo-Control of Enzyme-Collagen Membrane Activity

      • S. Suzuki, I. Karube, Y. Nakamoto, K. Namba

      Pages 163-166

   4. Enzyme Immobilization in Collagen Films Adhered to Semi-Rigid Supports: The Enzymatic Sponge Reactor

      • O. Maldonado, C. Rolz

      Pages 167-177

   5. Hollow Fiber Enzymatic Reactors: An Engineering Approach

      • B. R. Breslau, B. M. Kilcullen

      Pages 179-190

2. Enzyme Engineering and Enzyme Purification

   1. Front Matter

      Pages 191-191

      PDF

   2. Genetic Engineering with Nitrogen Fixation

      • K. T. Shanmugam, C. Morandi, K. Andersen, R. C. Valentine

      Pages 193-205

   3. The State of Enzyme Isolation Technology

      • P. Dunnill

      Pages 207-215

   4. Unit Operations of Enzyme Purification

      • C. W. Hancher

      Pages 217-223

   5. Scale-Up of Enzyme Purification

      • Henry R. Bungay

      Pages 225-233

   6. Carbon Dioxide as a Reagent for Proteins

      • M. A. Mitz

      Pages 235-239

   7. Application of Computers to Enzyme Recovery

      • R. P. Jefferis III, M. R. Kula

      Pages 241-248

   8. Studies on Immobilized Trypsin in High Concentrations of Organic Solvents

      • H. Weetall, P. Vann

      Pages 249-254

   9. A Tapered Fluidized Bed as a Bioreactor

      • C. D. Scott, C. W. Hancher, S. E. Shumate II

      Pages 255-261

   10. Induction and Purification of Phenol Hydroxylase from Tricosporon Cutaneum

       • J. R. Frederick, E. K. Pye

       Pages 263-271

3. Affinity Chromatography and Enzyme Cofactor Immobilization, Application and Regeneration

   1. Front Matter

      Pages 273-273

      PDF

   2. Analysis of Ligand-Macromolecule Interactions by Quantitative Affinity Chromatography

      • I. M. Chaiken

      Pages 275-282

   3. Affinity Chromatography: Interferences and Their Prevention

      • M. Wilchek

      Pages 283-289

   4. Immobilized Cofactors and Cofactor Fragments in General Ligand Affinity Chromatography and as Active Cofactors

      • K. Mosbach, P.-O. Larsson

      Pages 291-298

   5. Purification of Dehydrogenases and Kinases by Affinity Chromatography

      • C.-Y. Lee, L. H. Lazarus, N. O. Kaplan

      Pages 299-311

The recent worldwide explosion of interest in enzymes as cata­ lysts in industrial processes has arisen primarily because of the potential of major innovative advances which have taken place over the last two decades, foremost among these being novel methods of enzyme immobilization and affinity chromatography for rapid enzyme purification. This interest is now being further stimulated by the remarkable commercial success of several enzyme-based industrial processes, particularly the production of high-fructose syrup in the U. S. and amino acid production in Japan. With the initiation of these and other processes, together with the readying for commercial­ ization of several other enzyme-based operations, interest has ex­ panded in other areas in which enzymes may playa useful role, particularly in medicine and analytical chemistry. The development of this technology has required the cooperative efforts of practi­ tioners of several disciplines, primarily chemical engineers, bio­ chemists and other life scientists. Indeed, from this cooperation is arising the new interdisciplinary field of Enzyme Engineering. To stimulate communication, information exchange and advance­ ment of knowledge in this new field on an international level the Engineering Foundation, through the efforts of Lemuel B. Wingard, Jr. , initiated in 1971 a series of international conferences on Enzyme Engineering to be held biannually. The first two conferences were held in Henniker, New Hampshire, in the summers of 1971 and 1973, respectively, while the third conference, from which these proceedings derived, was held in August 1975 in Portland, Oregon.

• Glucose
• amino acid
• biotechnology
• chemistry
• chromatography
• communication
• development
• engine
• enzyme
• enzymes
• genetic engineering
• induction
• production
• protein
• yeast

• Department of Biochemistry and Biophysics School of Medicine, University of Pennsylvania, Philadelphia, USA

  E. Kendall Pye

• Research and Development Labs, Corning Glass Works, Corning, USA

  Howard H. Weetall

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