Methods of Protein Separation

  • Nicholas Catsimpoolas

Part of the Biological Separations book series (BIOSEP)

Table of contents

  1. Front Matter
    Pages I-XIII
  2. Nicholas Catsimpoolas
    Pages 27-67
  3. Alfred J. Crowle
    Pages 69-92
  4. James W. Drysdale
    Pages 93-126
  5. Robert E. Feeney, David T. Osuga
    Pages 127-160
  6. Eli Grushka
    Pages 161-192
  7. Burton A. Zabin
    Pages 239-254
  8. Indu Parikh, Pedro Cuatrecasas
    Pages 255-276
  9. Back Matter
    Pages 277-281

About this book


This open-end treatise on methods concerning protein separation had its beginning in an American Chemical Society symposium entitled "Con­ temporary Protein Separation Methods" which was held in Atlantic City, New Jersey in September 1974. The purpose of the symposium-and subse­ quently of the present work-was to review the available modern techniques and underlying principles for achieving one of the very important tasks of experimental biology, namely the separation and characterization of proteins present in complex biological mixtures. Physicochemical characterization was covered only as related to the parent method of fractionation and there­ fore involved mostly mass transport processes. Additionally, the presentation of methods for gaini. ng insight into complex interacting protein profiles was considered of paramount importance in the interpretation of separation patterns. Finally, specific categories of proteins (e. g. , chemically modified, deriving from a specific tissue, conjugated to different moieties, etc. ) require meticulous trial and selection andjor modification of existing methodology to carry out the desired separation. In such cases, the gained experience provides valuable guidelines for further experimentation. Although powerful techniques exist today for the separation and related physicochemical characterization of proteins, many biological fractionation problems require further innovations. It is hoped that the description in the present treatise of some of the available separation tools and their limitations will provide the necessary integrated background for new developments in this area.


amino acid biological biology development enzyme enzymes experiment ferritin nucleic acid protein proteins society tissue transport

Editors and affiliations

  • Nicholas Catsimpoolas
    • 1
  1. 1.Biophysics Laboratory, Department of Nutrition and Food ScienceMassachusetts Institute of TechnologyCambridgeUSA

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