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Pharmaceutical Applications of Cell and Tissue Culture to Drug Transport

  • Glynn Wilson
  • S. S. Davis
  • L. Illum
  • Alain Zweibaum

Part of the NATO ASI Series book series (NSSA, volume 218)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Ronald T. Borchardt, Ismael J. Hidalgo, Kathleen M. Hillgren, Ming Hu
    Pages 1-14
  3. Alain Zweibaum
    Pages 27-37
  4. Ismael J. Hidalgo, Ronald T. Borchardt
    Pages 77-91
  5. Per Artursson, Johan Karlsson
    Pages 93-105
  6. H. Leuenberger, S. Buchmann, C. Reinke, B. Schmid
    Pages 121-139
  7. Christian Huet, Odile Godefroy, Cristina Ibarra, Alice Dautry-Varsat, Daniel Louvard
    Pages 141-154
  8. Christian L. Laboisse
    Pages 155-161
  9. Kenneth L. Audus
    Pages 163-174
  10. Joost B. M. M. van Bree, Albertus G. de Boer, Meindert Danhof, Douwe D. Breimer
    Pages 175-189
  11. Stéphane Méresse, Marie-Pierre Dehouck, Pierre Delorme, Jean-Charles Fruchart, Roméo Cecchelli
    Pages 217-229
  12. Richard C. Boucher, Elaine H. C. Cheng
    Pages 249-258
  13. Dirk Breitkreutz, Petra Boukamp, Andrea Hülsen, Cathy Ryle, Hans-Jürgen Stark, Hans Smola et al.
    Pages 283-296
  14. Nydia G. Testa, Thomas M. Dexter, Terence D. Allen
    Pages 297-306
  15. Gerrit L. Scherphof, Toos Daemen, Johannes T. P. Derksen
    Pages 321-330
  16. Menemse Kiremitci, Ismet Gurhan, Erhan Piskin
    Pages 343-346
  17. G. Wilson, S. S. Davis, L. Illum, A. Zweibaum
    Pages 375-378
  18. Back Matter
    Pages 379-387

About this book

Introduction

In recent years there have been rapid advances in the growth and differentiation of mammalian cells in culture. This has led to increasing use of such in vitro systems in a wide variety of studies on fundamental aspects of cell structure and function, including normal growth and metabolism, mechanisms of differentiation and oncogenesis, mechanisms of protein and membrane synthesis and cell polarity. Recent advances in our ability to grow cells, including human cells, on permeable supports, to generate confluent cellular barriers with the morphological polarity corresponding to their in vivo counterparts has greatly facilitated such studies. In particular these new techniques have led to an increasing interest in the use of cell and tissue culture systems as a means for examining the transport of drugs across epithelial and endothelial barriers. An obvious question is whether these new in vitro methodologies will provide convenient systems that can substitute for and replace animal models. Various research groups both in academia and in the pharmaceutical industry have been investigating these types of methodologies in order to develop convenient well characterized systems that can be used to examine basic aspects of transcellular transport and to evaluate the permeability of drug molecules and delivery systems. Of particular note is use of confluent cell layers to study the transport of large molecules such as peptides and proteins produced through recombinant DNA technology.

Keywords

Colon DNA Mammalia cell culture drug growth metabolism oncogene pharmaceutical protein proteins receptor synthesis tissue transport

Editors and affiliations

  • Glynn Wilson
    • 1
  • S. S. Davis
    • 2
  • L. Illum
    • 2
  • Alain Zweibaum
    • 3
  1. 1.SmithKline Beecham PharmaceuticalsKing of PrussiaUSA
  2. 2.University of NottinghamNottinghamUK
  3. 3.INSERMParisFrance

Bibliographic information

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