Separations Using Aqueous Phase Systems

Applications in Cell Biology and Biotechnology

  • Derek Fisher
  • Ian A. Sutherland

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Partitioning of Macromolecules

    1. Front Matter
      Pages 1-1
    2. Göte Johansson
      Pages 7-14
    3. Gerd Birkenmeier, Per-Ake Ålbertsson, Gerhard Kopperschläger
      Pages 15-23
    4. Gerhard Kopperschläger, Jürgen Kirchberger, Thomas Kriegel
      Pages 41-42
  3. Partitioning of Plant Membranes and Organelles

  4. Partitioning of Animal Membranes and Organelles

    1. Front Matter
      Pages 65-65
    2. Austin K. Mircheff, Michael E. Bradley, Charles B. Hensley, Emile J. J. M. van Corven, Samuel C. Yiu, Ross W. Lambert
      Pages 67-80
    3. Peter Gierow, Bengt Jergil, Per-Åke Albertsson
      Pages 101-107
    4. D. David Nowack, Dorothy M. Morré, D. James Morré
      Pages 109-110
    5. Dorothy M. Morré, D. James Morré, Timothy Snyder, William Li, Amy Erb, David W. Sammons
      Pages 113-115
  5. Partitioning of Cells

    1. Front Matter
      Pages 117-117
    2. Claes-Goran Axelsson
      Pages 127-135
    3. D. J. Watts, Gillian M. Knight, J. Lawry, P. T. Sharpe
      Pages 157-164
    4. Paul Eggleton, Derek Fisher, Neville Crawford
      Pages 167-168
    5. C. P. S. Tilcock, T. Dempsey, P. R. Cullis, D. Fisher
      Pages 179-180
  6. Immuno—affinity Partitioning

    1. Front Matter
      Pages 181-181
    2. S. Jill Stocks, Donald E. Brooks
      Pages 183-191
    3. Laurel J. Karr, James M. Van Alstine, Robert S. Snyder, Steven G. Shafer, J. Milton Harris
      Pages 193-202
    4. J. M. Harris, K. Yoshinaga, M. S. Paley, M. R. Herati
      Pages 203-210
  7. Theoretical Aspects of Phase Partitioning

    1. Front Matter
      Pages 215-215
    2. T. E. Treffry, T. H. Lilley, P. J. Cheek
      Pages 233-238
    3. Samuel Schürch, Nigel A. M. Paterson, Donald J. L. McIver
      Pages 249-259
    4. Donald J. L. McIver, Samuel Schürch
      Pages 261-270
    5. Bernadette N. Youens, W. David Cooper, Frank D. Raymond, Paul S. Gascoine, Derek Fisher
      Pages 271-280

About this book


The use of aqueous two-phase systems for the partitioning of macromolecules, organelles and cells was originally developed by Per-Ake Albertsson in Sweden in the mid-fifties [1-3]. These systems were initially applied to separations of plant organelles and viruses but their use has now extended into most areas of cell biology and biochemistry [4,5]. Since 1979 biennial International Conferences on Partitioning in Aqueous Two-Phase Systems have been held in Los Angeles (1979), Sheffield (1981), Vancouver (1983) and Lund (1985). The 5th conference was held in Oxford from 23-28 August 1987 and was entitled "Advances in Separations Using Aqueous Phase Systems in Cell Biology and Biotechnology". It is the formal presentations from this meeting which comprise this volume. In contrast to earlier books on phase partitioning [4,5] this volume contains, for the first time, worldwide contributions from over sixty partitioners from a variety of scientific disciplines, thereby providing a detailed overview of the widespread application and potential of bioseparations using phase partitioning. Disciplines include Biophysics, Biochemistry, Cell Biology, Microbiology, Biotechnology and Process Engineering, in both academic and commercial establishments. These biennial conferences allow advances in these diverse partitioning fields to be reviewed and compared; they also provide an opportunity for those considering using phase partitioning to obtain information, advice and contacts. Attendance has grown steadily over the years and 140 scientists came to Oxford. The conference consisted of ten symposia on areas of application of partitioning which have been organised as specific chapters in this volume.


Lipid biochemistry biology biophysics cell biology cell culture enzyme enzymes polysaccharide protein receptor

Editors and affiliations

  • Derek Fisher
    • 1
  • Ian A. Sutherland
    • 2
  1. 1.Royal Free Hospital School of MedicineLondonUK
  2. 2.National Institute for Medical ResearchLondonUK

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