Human Hybridomas and Monoclonal Antibodies

  • Edgar G. Engleman
  • Steven K. H. Foung
  • James W. Larrick
  • Andrew A. Raubitschek

Table of contents

  1. Front Matter
    Pages i-xxiii
  2. B-Cell Lines, Hybridomas, and Monoclonal Antibodies

    1. Front Matter
      Pages 1-1
    2. Background and General Strategies

      1. Danuta Kozbor, Carlo M. Croce
        Pages 21-36
      2. John C. Roder, Susan P. C. Cole, Tsehay Atlaw, Barbara G. Campling, Ronald C. McGarry, Danuta Kozbor
        Pages 55-70
      3. Gregory R. Reyes, Marcia Bieber, Kirk E. Fry, Kit S. Lam, Joan M. Hebert, Nelson N. H. Teng
        Pages 71-91
    3. Applications to Infectious Diseases

      1. Warren C. Bogard Jr., Elizabeth Hornberger, Patrick C. Kung
        Pages 95-112
      2. Karen G. Burnett, Julia P. Leung, Joanne Martinis
        Pages 113-133
      3. Steven K. H. Foung, Susan Perkins, Jeffrey Lifson, Nahid Mohagheghpour, Dianne Fishwild, F. Carl Grumet et al.
        Pages 135-148
      4. James W. Larrick, Bradley J. Dyer, George Senyk, Sarah M. Hart, Richard Moss, David Lippman et al.
        Pages 149-165
      5. Anthony W. Siadak, Mark E. Lostrom
        Pages 167-185
    4. Applications to Cancer

      1. Mark C. Glassy, Harold H. Handley, Ivor Royston
        Pages 211-225
      2. Lennart Olsson, Peter Brams
        Pages 227-244
    5. Applications to Autoimmunity

      1. Yehuda Shoenfeld, Robert S. Schwartz
        Pages 247-262
      2. Floyd Taub, Jo Satoh, Carlo Garzelli, Karim Essani, Abner Louis Notkins
        Pages 263-275
      3. Michael K. Hoffmann, John A. Hirst
        Pages 277-289
    6. Special Topics

      1. Kathleen A. Denis, Randolph Wall, Andrew Saxon
        Pages 293-307
  3. Human T-T Hybridomas

    1. Front Matter
      Pages 309-309
    2. Steven Coutre, Steven K. H. Foung, Edgar G. Engleman
      Pages 311-321
    3. C. Gravekamp, S. J. L. Bol, A. Hagemeijer, R. L. H. Bolhuis
      Pages 323-339
    4. Joseph L. Butler, Julian L. Ambrus Jr., Anthony S. Fauci
      Pages 341-353
    5. Toshiaki Osawa, Yoshiro Kobayashi, Makoto Asada, Masahiro Higuchi, Shu-ichi Tsuchiya
      Pages 371-384
    6. Mary A. Valentine, Dennis A. Carson
      Pages 385-400
  4. Back Matter
    Pages 419-526

About this book


Soon after Kohler and Milstein described the use of somatic cell hybridization for the production of murine monoclonal antibodies of desired specificity, this relatively simple technique became widely applied. Indeed, production of murine monoclonal antibodies is now considered routine by immunologists and nonimmunologists alike. However, as heterologous proteins, mouse monoclonal antibodies have one major limitation: they are immunogenic in man and, hence, their use in vivo is severely limited. An obvious solution to this problem is to produce human hybridomas with the same techniques used for the production of rodent hybrids. Unfortunately, the history of human hybridomas has been marked by substantive and often exasperating tech­ nical problems, and the first reports of hybrids secreting human immu­ noglobulin of desired specificity did not appear until 1980. These reports were met with initial enthusiasm, but it soon became apparent that while human lymphocytes might be fused, their frequency, level of Ig synthesis, and stability were such that production of human antibodies with this method was neither routine nor practical. Nonetheless, a sufficient number of investiga­ tors persevered, and during the next 5 years relatively efficient B-cell fusion partners as well as improved methods of Epstein-Barr virus transformation were developed. Generation of human T -T hybrids has also been achieved, although problems of chromosomal stability remain a substantial obstacle, more so than with B-cell lines.


antibody lymphocytes proteins virus

Editors and affiliations

  • Edgar G. Engleman
    • 1
  • Steven K. H. Foung
    • 1
  • James W. Larrick
    • 2
  • Andrew A. Raubitschek
    • 2
  1. 1.Department of Pathology, Stanford University School of MedicineStanford University Medical CenterStanfordUSA
  2. 2.Cetus Immune Research LaboratoriesPalo AltoUSA

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