© 1986

In Situ Hybridization in Brain

  • George R. Uhl

Table of contents

  1. Front Matter
    Pages i-xx
  2. In Situ Hybridization Approaches and Applications

    1. Front Matter
      Pages 1-1
    2. Brenda D. Shivers, Richard E. Harlan, Gary J. Romano, Richard D. Howells, Donald W. Pfaff
      Pages 3-20
    3. George R. Uhl, Jacqueline Evans, Mark Parta, Charles Walworth, Kelly Hill, Cathrine Sasek et al.
      Pages 21-47
    4. Thomas G. Sherman, Jeffrey E. Kelsey, Henry Khachaturian, Sharon Burke, Huda Akil, Stanley J. Watson
      Pages 49-62
    5. Ruth E. Siegel, W. Scott Young III
      Pages 63-71
    6. Frank Baldino Jr., Leonard G. Davis
      Pages 97-116
    7. Michael C. Wilson, Gerald A. Higgins, Hartwig Schmale, Floyd E. Bloom, Robert J. Milner
      Pages 117-133
    8. Carol W. Wuenschell, Robin S. Fisher, Niranjala J. K. Tillakaratne, Allen J. Tobin
      Pages 135-149
    9. Michel Goedert, Stephen P. Hunt, Paul D. Rennert, Gerhard Heinrich
      Pages 151-169
    10. Donald L. Price, Paul N. Hoffman, Richard J. Altschuler, Edward H. M. Koo, Peter J. Whitehouse, John W. Griffin et al.
      Pages 171-180
    11. J. Stephen Fink, Marc R. Montminy, Toshihiko Tsukada, Heinz Hoefler, Linda A. Specht, Ronald M. Lechan et al.
      Pages 181-191
    12. Howard E. Gendelman, Scott Koenig, Allen Aksamit, Sundarajan Venkatesan, Wallace W. Tourtellotte, Peter Schmid et al.
      Pages 203-223
  3. Methodological Issues

    1. Front Matter
      Pages 225-225
    2. George Uhl, S. Watson, J. Kelsey, Michel Goedert, W. Scott Young III
      Pages 227-238
    3. Duncan J. Campbell
      Pages 239-242

About this book


The explosion of interest in specific molecules important for brain function and dysfunction has drawn individuals from diverse backgrounds toward the use of in situ hybridization techniques. Study of the brain demands the anatomic precision and biochemical specificity that this approach can potentially bring. Workers with backgrounds in peptide neuroanatomy, neuropharmacology, molecular biology, neurovirology, neuropathology, and neurophysiology have joined together in this volume to discuss their initial experiences in applying ill situ hybridization techniques to the study of the brain. The work, although still in an early phase of development, is worthy of initial summary and dissemination. In the area of neuropeptide gene expression alone, investigators represented here describe studies of vasopressin, opiate peptides, oxytocin, vasoactive intestinal peptide, cholecystokinin, and somatostatin. Other contributions provide insight into applications of the technique to studies of the expression of genes for neurotransmitter synthesizing enzymes, viral-encoded genes, trophic factor genes, and the genes selected on the basis of their special roles in the brain. The authors provide an important series of technical perspectives, and describe specific experimental protocols. This volume should be of interest to individuals seeking an introduction to these methods, as well to those desiring an up to date precis of work in this burgeoning area. Dr. Uhl, with the sponsorship of the Howard Hughes Medical Institute, has done a superb job of assembling the leaders in this area, and in organizing the presen ta tion of their perspecti ves herein. Joseph B. Martin, M.D., Ph.D.


Europe anatomy biology brain experiment growth molecular biology nervous system neuroanatomy neuropathology neuropeptides neuropharmacology neurophysiology physiology

Editors and affiliations

  • George R. Uhl
    • 1
    • 2
  1. 1.Department of Neurology and Howard Hughes Medical InstituteUSA
  2. 2.Massachusetts General Hospital and Harvard Medical SchoolBostonUSA

Bibliographic information