Eukaryotic Cell Cultures

Basics and Applications

  • Ronald T. Acton
  • J. Daniel Lynn

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 172)

Table of contents

  1. Front Matter
    Pages i-x
  2. Establishing, Characterizing and Growing Cells in Culture

    1. Bharati Hukku, David M. Halton, Michael Mally, Ward D. Peterson Jr.
      Pages 13-31
    2. Dennis A. Stewart, Robert B. Bolin, Barbara A. Cheney, Joseph T. Hawkins, Keith W. Chapman, Donald R. Tompkins
      Pages 33-48
    3. Judith B. Grinspan, Stephen N. Mueller, James P. Noveral, Eliot M. Rosen, Elliot M. Levine
      Pages 67-90
    4. Evelyn W. Jemison, Kenneth Brown, Beverly Rivers, Regina Knight
      Pages 91-117
    5. Nikos K. Harakas, Charles Lewis, Ronald D. Bartram, Bernard S. Wildi, Joseph Feder
      Pages 119-138
    6. Christine Gebb, Julian M. Clark, Michael D. Hirtenstein, Göran E. Lindgren, Bjöorn J. Lundgren, Ulla Lindskog et al.
      Pages 151-167
  3. Products of Cells in Culture

    1. Corinne M. Gajdusek, Sandra A. Harris-Hooker
      Pages 179-203
    2. G. A. Granger, J. Klostergaard, R. S. Yamamoto, J. Devlin, S. L. Orr, D. McGriff et al.
      Pages 205-217
    3. Jerome E. Groopman, Ilana Nathan, David W. Golde
      Pages 219-225
    4. Dale F. Gruber, G. David Ledney
      Pages 227-240
    5. Marian L. Lewis, Dennis R. Morrison, Bernard J. Mieszkuc, Diane L. Fessler
      Pages 241-267
  4. Use of Recombinant DNA Technology

  5. Lymphocytes in Culture: A System for Understanding Immune Responsiveness

    1. Frank W. Fitch
      Pages 347-363
    2. Oscar Irigoyen, Philip V. Rizzolo, Yolene Thomas, Linda Rogozinski, Leonard Chess
      Pages 383-404
    3. Dolores J. Schendel, Rudolf Wank
      Pages 405-418
  6. Use of Cells in Culture to Study Disease

    1. Euguene Bell, Stephanie Ellsworth Sher, Barbara E. Hull, Robert L. Sarber, Seymour Rosen
      Pages 419-433
    2. Thomas F. Bumol, John R. Harper, Darwin O. Chee, Ralph A. Reisfeld
      Pages 455-470
    3. Henry C. Lyko, James X. Hartmann
      Pages 471-488
    4. Collin J. Weber, F. Xavier Pi-Sunyer, Earl Zimmerman, Gajanan Nilaver, Michael Kazim, Orion Hegre et al.
      Pages 489-525
    5. Gerald Weissmann, Charles Serhan, Helen M. Korchak, James E. Smolen
      Pages 527-552
  7. Back Matter
    Pages 553-556

About this book


The Second International Cell Culture Congress was structured as was the First Congress to bring together scientists from academia and industry to discuss the use of cell culture in support of bioscience. It was felt that a forum whereby state-of­ the-art presentations were followed by informal workshops would provide opportunity for the greatest exchange of information. Within the atmosphere of the workshop, problems common to basic as well as applied research were discussed and directions for the future were brought to light. These proceedings reflect and epitomize those discussions. Although it is difficult to cover all scientific disciplines utilizing cells in culture, we feel key areas were addressed at the Congress and are herein presented. Considerable emphasis has been given to the methods for establishing cells in culture and characterizing the cells once established as well as the improved technology for growing established cell lines. Examples of how recombinant DNA technology is being used to manipulate genes within mammalian cells, to clone mammalian genes and to insert them in prokaryotes has been included. Major emphasis has been given to the use of lymphocytes in culture for understanding immune responsiveness and the culturing of a variety of cell types as a means to understand disease states.


DNA cell cell culture cells endothelium glycoprotein interferon leukemia leukemia virus molecular genetics neutrophils physiology proteins smooth muscle tissue

Editors and affiliations

  • Ronald T. Acton
    • 1
  • J. Daniel Lynn
    • 1
  1. 1.Department of MicrobiologyUniversity of Alabama in BirminghamBirminghamUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 1984
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4615-9378-2
  • Online ISBN 978-1-4615-9376-8
  • Series Print ISSN 0065-2598
  • Series Online ISSN 2214-8019
  • Buy this book on publisher's site
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