Mammalian Cell Culture

The Use of Serum-Free Hormone-Supplemented Media

  • Jennie P. Mather

Table of contents

  1. Front Matter
    Pages i-xvii
  2. W. J. Pledger, J. E. Estes, P. H. Howe, E. B. Leof
    Pages 1-15
  3. Theodore R. Breitman, Beverly R. Keene, Hiromichi Hemmi
    Pages 77-101
  4. Izumi Hayashi, Brian I. Carr
    Pages 151-166
  5. Jennie P. Mather
    Pages 167-193
  6. David Barnes
    Pages 195-237
  7. Back Matter
    Pages 281-284

About this book


The advantages of obtaining a completely defined environment for the growth of cells in vitro were recognized very early in the history of cell culture (Lewis and Lewis, 1911). Continued interest in the nutritional requirements of cells in vitro and in providing an optimal environment for cells led to the development of the complex nutrient mixtures available today in many media (Waymouth, 1972; Ham, 1965). However, serum remained an essential component of medium for the growth of most cell types in culture. The question of what factor (or factors) in serum was essential for cell growth and survival remained unanswered for several decades. Initially, experiments were designed to purify the "active component" of serum for the growth of cells in culture. These experiments identified fetuin (Fisher et at., 1958) and nonsuppressible insulinlike activity (Temin et at., 1972) as important components of serum. However, the complexity of serum and the very low levels of active components in serum hindered progress in identi­ fying and isolating serum factors.


cell cell culture cells complexity development growth insulin kidney nutrition

Editors and affiliations

  • Jennie P. Mather
    • 1
    • 2
  1. 1.The Population CouncilNew YorkUSA
  2. 2.The Rockefeller UniversityNew YorkUSA

Bibliographic information

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