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Single-Channel Recording

  • Bert Sakmann
  • Erwin Neher
Book

Table of contents

  1. Front Matter
    Pages i-xxii
  2. Methods

    1. Front Matter
      Pages 1-1
    2. F. J. Sigworth
      Pages 3-35
    3. D. P. Corey, C. F. Stevens
      Pages 53-68
    4. David W. Tank, Christopher Miller
      Pages 91-105
    5. Alain Marty, Erwin Neher
      Pages 107-122
    6. Walter Stühmer, William M. Roberts, Wolfhard Almers
      Pages 123-132
  3. Concepts and Analysis

    1. Front Matter
      Pages 133-133
    2. D. Colquhoun, F. J. Sigworth
      Pages 191-263
    3. Frederick Sachs
      Pages 265-285
    4. Richard W. Aldrich, Gary Yellen
      Pages 287-299
    5. F. J. Sigworth
      Pages 301-321
    6. Louis J. DeFelice, John R. Clay
      Pages 323-342
  4. Patch Clamp Data

  5. Back Matter
    Pages 481-503

About this book

Introduction

Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an introductory chapter, we chose to reprint that article in the Appendix of this volume (by permission of Springer-Verlag). The methods section will, therefore, go straight into detailed aspects of the technol­ ogy.

Keywords

activation art cell cell culture enzyme influence liposomes membrane muscle neurons resistance time tissue

Editors and affiliations

  • Bert Sakmann
    • 1
  • Erwin Neher
    • 1
  1. 1.Max-Planck-Institut für biophysikalische ChemieGöttingenFederal Republic of Germany

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