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Novel Methods in Molecular and Cellular Biochemistry of Muscle

  • Grant N. Pierce
  • William C. Claycomb
Book

Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 20)

Table of contents

  1. Front Matter
    Pages i-1
  2. Michael J. Mann, Ryuichi Morishita, Gary H. Gibbons, Heiko E. von der Leyen, Victor J. Dzau
    Pages 3-12
  3. Hiroaki Ooboshi, C. David Ríos, Donald D. Heistad
    Pages 37-46
  4. R. Daniel Gietz, Anne Robbins, Kevin C. Graham, Barbara Triggs-Raine, Robin A. Woods
    Pages 67-79
  5. C. C. Liew, D. M. Hwang, R. X. Wang, S. H. Ng, A. Dempsey, D. H. Y. Wen et al.
    Pages 81-87
  6. R. Hullin, F. Asmus, G. Steinbeck
    Pages 89-95
  7. Michael P. Czubryt, Bram Ramjiawan, Grant N. Pierce
    Pages 97-102
  8. Manuela Bartoli, William C. Claycomb
    Pages 103-109
  9. Stephania Cormier-Regard, Daniel B. Egeland, Vivien J. Tannoch, William C. Claycomb
    Pages 111-120
  10. Alba Vecchini, Luciano Binaglia, Vincenzo Panagia
    Pages 129-136
  11. Yan-Jun Xu, Qiming Shao, Naranjan S. Dhalla
    Pages 149-157
  12. James S. C. Gilchrist, Chris Palahniuk, Ratna Bose
    Pages 159-170
  13. Ghassan Bkaily, Pierre Pothier, Pedro D’Orléans-Juste, May Simaan, Danielle Jacques, Doris Jaalouk et al.
    Pages 171-194
  14. P. D’Orléans-Juste, D. Regoli, L. H. Pheng, G. Bkaily, M. G. Sirois, E. R. Edelman et al.
    Pages 199-211
  15. W. Michael Flanagan, Richard W. Wagner
    Pages 213-225
  16. Back Matter
    Pages 239-241

About this book

Introduction

Experimental techniques are the life blood of science. The better the methodology is, the more reliable and accurate the results will be. Ultimately, this will lead to a clearer interpretation of those results and firmer conclusions from any set of experiments. Experimental methodology in the area of cardiovascular biochemistry and molecular biology has advanced considerably in the last decade. Because of these factors, it was thought that a focused issue of Molecular and Cellular Biochemistry dedicated to the novel, latest technological advances in the field was warranted. We must thank Dr Naranjan S. Dhalla, Editor-in-Chief of Molecular and Cellular Biochemistry, for his willingness to publish an issue with such a focus. We have attracted some of the leaders in the field of cardiovascular biology to submit articles describing some of the most novel, significant techniques currently in use in their laboratories. The purpose of the manuscripts was not to describe the recent experimental findings from each laboratory as is done in most conventional manuscripts. Instead, the purpose of the articles found within this focused volume of Molecular and Cellular Biochemistry was to describe how the technique is performed on the laboratory bench so that others less familiar with the technique may be able to use it in their own labs. The subjects described in this volume can be generally subdivided into three categories: molecular biology, cell biology and basic biochemistry. The methods cover wide areas including various DNA and RNA expression technologies, transfection techniques, quantification of ion flux movement, measurements of lipid metabolism, advances in the culture of specific cardiovascular cell populations, and the use of confocal microscopy to examine cell structure and function. We thank all of the authors who have contributed so much of their time and efforts and, most importantly, shared the `secrets' of these valuable techniques with the rest of the cardiovascular research community.

Keywords

DNA Lipid Nucleotide RNA biochemistry gene expression metabolism

Editors and affiliations

  • Grant N. Pierce
    • 1
  • William C. Claycomb
    • 2
  1. 1.Institute of Cardiovascular SciencesSt. Boniface General Hospital Research CentreWinnipegCanada
  2. 2.Department of Biochemistry and Molecular BiologyLouisiana State University Medical CenterNew OrleansUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4615-6353-2
  • Copyright Information Springer Science+Business Media Dordrecht 1997
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4613-7918-8
  • Online ISBN 978-1-4615-6353-2
  • Buy this book on publisher's site
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