Identification of Transcribed Sequences

  • Ute Hochgeschwender
  • Katheleen Gardiner

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction: Seven Blind Men and an Elephant

  3. From Genomic DNA to Transcribed Sequences

    1. Classical Approaches

      1. C. Tribioli, E. Maestrini, S. Bione, F. Tamanini, M. Mancini, C. Sala et al.
        Pages 5-10
      2. Eric N. Burright, N. German Pasteris, Michael D. Bialecki, Jerome L. Gorski
        Pages 11-21
      3. Gregory G. Lennon, Kimberly Lieuallen
        Pages 23-28
    2. Hybridization Based Approaches

      1. Sankhavaram R. Patanjali, Hong Xia Xu, Satish Parimoo, Sherman M. Weissman
        Pages 29-35
      2. K. Gardiner, H. Xu, W. Bonds, F. Tassone, S. Parimoo, R. Sivakamasundari et al.
        Pages 37-49
      3. Fergus J. Couch, Barbara L. Weber, Francis S. Collins, Danilo A. Tagle
        Pages 51-63
      4. J. M. Rommens, L. Mar, J. McArthur, L.-C. Tsui, S. W. Scherer
        Pages 65-79
      5. Pudur Jagadeeswaran, Michael W. Odom, Edward J. Boland
        Pages 101-109
      6. J. C. Hozier, L. M. Davis, P. D. Siebert, K. Dietrich, M. C. Paterson
        Pages 123-138
      7. Wolfgang Schwabe, Brenda J. Lawrence, Adelaide S. Robb, Rene M. Hopfinger, Ute Hochgeschwender, Miles B. Brennan
        Pages 139-155
      8. Jeffrey D. Falk, Hiroshi Usui, J. Gregor Sutcliffe
        Pages 157-167
    3. Exon Trapping

      1. Nicole A. Datson, Geoffrey M. Duyk, Lau A. J. Blonden, Gert-Jan B. Van Ommen, Johan T. Den Dunnen
        Pages 169-181
      2. Kenneth J. Abel, Lucio H. Castilla, Alan J. Buckler, Fergus J. Couch, Peggy Ho, Ida Schaefer et al.
        Pages 183-198
      3. Michael North, Fernando Gibson, Stephen Brown, Beatrice Griffiths, Ellen Solomon, Hans Lehrach
        Pages 199-212
      4. Marie-Laure Yaspo, Philippe Sanséau, Dean Nizetic, Bernhard Korn, Annemarie Poustka, Hans Lehrach
        Pages 213-228
    4. Computer Based Approaches

  4. From Transcribed Sequences to Genomic Localization

    1. Generating and Sequencing cDNAs

      1. R. Drmanac, S. Drmanac, I. Labat, N. Stavropoulos
        Pages 239-251
      2. Sebastian Meier-Ewert, Joachim Rothe, Richard Mott, Hans Lehrach
        Pages 253-260
      3. Mark G. Erlander, Ana Dopazo, Pamela E. Foye, J. Gregor Sutcliffe
        Pages 261-271
    2. Mapping cDNAs

      1. Donald T. Moir, Ron Lundstrom, Peter Richterich, Xiaohong Wang, Maria Atkinson, Kathy Falls et al.
        Pages 289-297
      2. Katheleen Gardiner, Ute Hochgeschwender
        Pages 299-302
  5. Back Matter
    Pages 303-304

About this book


The Human Genome Project, an endeavor to map and sequence the entire human genome, has been in existence for almost seven years. One result of this effort has been the development of increasingly detailed genetic and physical maps spanning large regions of virtually every chromosome. Paralleling this has been the increasingly high resolution mapping of many &wnetic diseases. Together, these developments have facilitated the isolation of specific disease genes and are now motivating the construction of comprehensive transcriptional maps. This latter endeavor represents a new facet of the genome project, and as such requires the recognition and solution of a new set of problems, with the attendant development and application of a new set of techniques. The First International Workshop on the Identification of Transcribed Sequences in the Human Genome was held in 1991 and was attended by 23 investigators. Discussions at this meeting were largely devoted to defining the magnitude of the problem and describing the available techniques. A small number of laboratories reported the development of new techniques (at that time, for example, exon trapping, cDNA hybrid selection, direct cDNA screening, use of splice junction conserved sequences,etc.), but data were too limited to permit comparisons of their relative efficiencies.


Chromosom DNA YAC cloning genes hybridization transcription

Editors and affiliations

  • Ute Hochgeschwender
    • 1
  • Katheleen Gardiner
    • 2
  1. 1.National Institute of Mental HealthBethesdaUSA
  2. 2.Eleanor Roosevelt Institute for Cancer ResearchDenverUSA

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