Nanoscale Imaging of Synapses

New Concepts and Opportunities

  • U. Valentin Nägerl
  • Antoine Triller

Part of the Neuromethods book series (NM, volume 84)

Table of contents

  1. Front Matter
    Pages i-x
  2. Xiaobing Chen, Christine Winters, Rita Azzam, Alioscka A. Sousa, Richard D. Leapman, Thomas S. Reese
    Pages 1-21
  3. Shigeki Watanabe, M. Wayne Davis, Erik M. Jorgensen
    Pages 43-57
  4. Christian G. Specht, Ignacio Izeddin, Maxime Dahan
    Pages 75-91
  5. Nicholas A. Frost, Harold D. MacGillavry, Hsiangmin E. Lu, Thomas A. Blanpied
    Pages 93-123
  6. Nathan Hedrick, Ryohei Yasuda
    Pages 145-159
  7. Svetlana Korotchenko, Francesca Cella Zanacchi, Alberto Diaspro, Alexander Dityatev
    Pages 187-203
  8. Back Matter
    Pages 221-231

About this book


Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in microscopy have greatly improved our ability to image synapses at the nanoscale, even down to the level of single molecules.

Authored by leading practitioners and developers in the field, this volume focuses on the nanoscale analysis of the molecular and structural organization and dynamics of synapses of the central nervous system, utilizing superresolution (e.g. PALM, STORM, STED) and other advanced methods (e.g. EM tomography, optogenetics, FLIM). It explains the basic principles behind the various nanoscale imaging modalities, how they are implemented and what their scope and limitations are, while also highlighting several exciting new research opportunities for synapse research enabled by them.


live-cell fluorescence microsopy nanoscopy neurons and glia physiology synapses

Editors and affiliations

  • U. Valentin Nägerl
    • 1
  • Antoine Triller
    • 2
  1. 1.Interdisciplinary Institute for NeuroscienceUniversity of BordeauxBordeauxFrance
  2. 2.Institute of Biology at Ecole Normale SupérieureEcole Normale SupérieureParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4614-9178-1
  • Online ISBN 978-1-4614-9179-8
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site
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