Gene Manipulation in Plant Improvement

16th Stadler Genetics Symposium

  • J. P. Gustafson

Part of the Stadler Genetics Symposia Series book series (SGSS)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Glenn W. Burton
    Pages 1-15
  3. Donald N. Duvick
    Pages 17-31
  4. S. Rajaram, B. Skovmand, B. C. Curtis
    Pages 33-60
  5. Donald C. Rasmusson
    Pages 95-119
  6. P. S. Baenziger, D. T. Kudirka, G. W. Schaeffer, M. D. Lazar
    Pages 385-414
  7. E. C. Cocking
    Pages 415-425
  8. Michael D. Bennett
    Pages 469-502
  9. Carole P. Meredith
    Pages 503-528
  10. R. Appels, Lyndall B. Moran
    Pages 529-557
  11. Sharon R. Long
    Pages 559-576
  12. J. D. Rochaix, J. Erickson, M. Goldschmidt-Clermont, M. Schneider, J. M. Vallet
    Pages 577-603
  13. John R. Bedbrook
    Pages 627-636
  14. N. W. Simmonds
    Pages 637-654
  15. Back Matter
    Pages 655-668

About this book


The results obtained to date involving the use of in ~ methods to facilitate wide hybridization in plants are voluminous and impressive. The techniques of embryo culture, ovule culture, and in~ pollination and fertilization represent an extension of the normal sexual hybridization process. Successes recorded in obtaining hybrids stem largely from circumventing prezygotic or postzygotic hybridization barriers. Numerous recent successful hybridizations were possible because of the development of improved tissue and cell culture systems for crop plants and attention given to genotypes used in hybridization attempts. Interspecific and intergeneric hybridization utilizing the process of protoplast fusion will bypass the limits set by all sexual me'thods. In addition to combining complete genomes from two different species through protoplast fusion, this system affords unique opportunities for creating novel cytoplasmic combinations, transfer of individual chromosomes, transfer of cytoplasmic organelles, manipulation of male sterility, and for single gene transfer. Some caution must be noted with regard to the extent of hybridization possible between distantly related species. Although practically no limit exists to the physical fusion of protoplasts from widely divergent species, the restrictions imposed by somatic incompatibility have not been adequately addressed. Regeneration of plants from the protoplast or single heterokaryon level is still a major hurdle for many important crop species before somatic cell fusion can be exploited to produce interspecific and intergeneric hybrids. Identification and selection of hybrids is also a limitation to the efficient application of cell fusion methods.


Chromosom Evolution Mutation gene expression genes hybridization nitrogen wheat

Editors and affiliations

  • J. P. Gustafson
    • 1
  1. 1.USDA-ARSUniversity of MissouriColumbiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag US 1984
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-1-4612-9478-8
  • Online ISBN 978-1-4613-2429-4
  • Series Print ISSN 1568-1009
  • Buy this book on publisher's site
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