© 1986

Manipulation of Mammalian Development

  • Ralph B. L. Gwatkin

Part of the Developmental Biology book series (DEBO, volume 4)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Katherine Gordon, Frank H. Ruddle
    Pages 1-36
  3. James McGrath, Davor Solter
    Pages 37-55
  4. Bela J. Gulyas
    Pages 57-80
  5. W. C. D. Hare
    Pages 195-216
  6. Frank B. Kuzan, George E. Seidel Jr.
    Pages 249-278
  7. Back Matter
    Pages 383-388

About this book


Developmental biology has been transformed recently by discoveries in the fields of molecular biology, cell biology, and immunology. New ways of manip­ ulating mammalian development are uncovering control mechanisms and ena­ bling us to apply them in solving practical problems in animal production and human health. This book outlines some of these new manipulations and how they have contributed to the present state of developmental biology. Chapter 1 describes gene transfer by micro injection of cloned recombinant DNA into zygotes. Although the factors that affect transformation frequencies and integration sites are still unknown, such techniques offer a number of exciting prospects. Research models for human disease coula be artificially created and desirable characteristics in agricultural animals could be - hanced. . The theme of cell-to-cell transfer is continued in Chapters 2 and 3. Chapter 2 describes pronuclear transplantation by Sendai virus-induced fusion of the karyoplast with the enucleated embryo. Using this procedure, it has been dem­ onstrated that both male and female genomes are essential for normal develop­ ment, although the reason for this is not yet understood. Chapter 3 describes studies on the fusion of whole oocytes. .


Embryo Mammalia development developmental biology genes tissue

Editors and affiliations

  • Ralph B. L. Gwatkin
    • 1
  1. 1.The Cleveland Clinic FoundationClevelandUSA

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