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Subtilisin Enzymes

Practical Protein Engineering

  • Book
  • © 1996

Overview

Editors:
  1. Richard Bott

    1. Genecor International, Inc., South San Francisco, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Christian Betzel

    1. European Molecular Biology Laboratory, Hamburg, Germany

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 379)

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Table of contents (29 chapters)

  1. Biophysical Probes and Mutagenesis

    1. Inhibition of Proteinase K by Mercury

      • Alexander Müller, Wolfram Saenger
      Pages 183-189

  2. Protein Engineering od Subtilisin Enzymes

    1. Crystallographic Investigations of Subtilisin BPN’ Mutants Engineered for Studying Thermal Stability

      • J. D. Oliver, T. J. Rydel, L. C. Strickland
      Pages 191-201

    2. Structural and Functional Consequences of Engineering the High Alkaline Serine Protease PB92

      • J. M. van der Laan, O. Misset, L. J. S. M. Mulleners, G. Gerritse, H. N. Scheffers, D. J. van Schouwen et al.
      Pages 203-218

    3. Engineering Surface Charges in a Subtilisin

      • M. R. Egmond, W. P. Antheunisse, P. Ravestein, A. T. A. Mooren, J. de Vlieg
      Pages 219-228

    4. PH Dependence of the Catalytic Activity of a Subtilisin-Like Proteinase

      • Gudrun Lange, Christian Betzel, Keith Wilson, Sven Branner
      Pages 229-233

    5. An Investigation of the Savinase Water Channel: Implications of Cavity Mutations

      • Ole H. Olsen, Jan T. Pedersenn, Christian Betzel, Susanne Eschenburg, Sven Branner, Sven Hastrup
      Pages 235-241

    6. Substrate Specificity of Natural Variants and Genetically Engineered Intermediates of Bacillus Lentus Alkaline Proteases

      • Karl-Heinz Maurer, Martina Markgraf, Dean Goddette
      Pages 243-256

    7. Unusual Ligand Binding at the Active Site Domain of an Engineered Mutant of Subtilisin BL

      • C. Paech, D. W. Goddette, T. Christianson, C. R. Wilson
      Pages 257-268

    8. Studies on the Structure and Function of Subtilisin E by Protein Engineering

      • Hiroshi Takagi, Hiroshi Matsuzawa, Takahisa Ohta, Makari Yamasaki, Masayori Inouye
      Pages 269-275

    9. Structural Changes Leading to Increased Enzymatic Activity in an Engineered Variant of Bacillus Lentus Subtilisin

      • Richard Bott, Judy Dauberman, Lori Wilson, Grant Ganshaw, Helena Sagar, Thomas Graycar et al.
      Pages 277-283

  3. Back Matter

    Pages 285-288
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Keywords

About this book

Subtilisin is the most extensively studied model system for protein engineering. The primary motivating factor for the interest in subtilisin is the commercial utility of this class of proteases. The subtilisin symposium was the first international meeting to bring together a large number of groups that have focused on the subtilisins and the subtilases-the protein superfamily of subtilisin-like enzymes. The results presented at the symposium are in this way a unique compendium of a broad spectrum of work largely focused on harnessing the potential of site-directed mutagenesis to understand and deliberately alter the function of these enzymes toward a desired end. This sort of protein engineering has been extremely successful in subtilisin, with many such "engineered" enzymes now widely used in commer­ cial enterprises. In this regard the experience derived from subtilisin does represent practical protein engineering. It is becoming clear that subtilisin represents a larger class of enzymes, the subtilases, that include many of the human pro hormone-converting enzymes. As international collabo­ rative efforts to sequence entire genomes continue, we can reasonably expect that additional members of the subtilase class will be encountered. Whenever interest in a member of this class of enzyme arises, the work on subtilisin will serve as a guide to the analysis for what in bacillus, fungi, and industry is an everyday workhorse enzyme.

Editors and Affiliations

  • Genecor International, Inc., South San Francisco, USA

    Richard Bott

  • European Molecular Biology Laboratory, Hamburg, Germany

    Christian Betzel

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