© 1996

Subtilisin Enzymes

Practical Protein Engineering

  • Richard Bott
  • Christian Betzel

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 379)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Structural Studies of Subtilisin Enzymes Alone and in Complex with Inhibitors

    1. Zbigniew Dauter, Christian Betzel, Nicolay Genov, Keith S. Wilson
      Pages 1-4
    2. Alexei Teplyakov, Piet Gros, Wim G. J. Hol
      Pages 5-9
    3. T. P. Singh, B. Padmanabhan, P. Narula, A. K. Saxena, C. Betzel, P. Sharma et al.
      Pages 11-20
    4. Sabine Pfeffer-Hennig, Zbigniew Dauter, Michael Hennig, Wolfgang Höhne, Keith Wilson, Christian Betzel
      Pages 29-41
    5. Christian Betzel, Silke Klupsch, Sven Branner, Keith S. Wilson
      Pages 49-61
  3. New Enzymes

  4. Structure-Function Studies

    1. Hanne Grøn, Lene M. Bech, Steen Bech Sørensen, Morten Meldal, Klaus Breddam
      Pages 105-112
    2. A. M. Wolff, M. S. Showell, M. G. Venegas, B. L. Barnett, W. C. Wertz
      Pages 113-120
    3. B. L. Barnett, Cathleen B. Turner
      Pages 121-131
  5. Biophysical Probes and Mutagenesis

    1. K. Peters, D. Brömine, G. Jahreis, S. Fittkau
      Pages 133-140
    2. Nicolay Genov, Peter Nikolov, Christian Betzel, Keith Wilson
      Pages 141-145
    3. Gary L. Gilliland, D. Travis Gallagher, Patrick Alexander, Philip Bryan
      Pages 159-169

About this book


Subtilisin is the most extensively studied model system for protein engineering. The primary motivating factor for the interest in subtilisin is the commercial utility of this class of proteases. The subtilisin symposium was the first international meeting to bring together a large number of groups that have focused on the subtilisins and the subtilases-the protein superfamily of subtilisin-like enzymes. The results presented at the symposium are in this way a unique compendium of a broad spectrum of work largely focused on harnessing the potential of site-directed mutagenesis to understand and deliberately alter the function of these enzymes toward a desired end. This sort of protein engineering has been extremely successful in subtilisin, with many such "engineered" enzymes now widely used in commer­ cial enterprises. In this regard the experience derived from subtilisin does represent practical protein engineering. It is becoming clear that subtilisin represents a larger class of enzymes, the subtilases, that include many of the human pro hormone-converting enzymes. As international collabo­ rative efforts to sequence entire genomes continue, we can reasonably expect that additional members of the subtilase class will be encountered. Whenever interest in a member of this class of enzyme arises, the work on subtilisin will serve as a guide to the analysis for what in bacillus, fungi, and industry is an everyday workhorse enzyme.


enzyme enzymes fungi genes protein protein engineering system

Editors and affiliations

  • Richard Bott
    • 1
  • Christian Betzel
    • 2
  1. 1.Genecor International, Inc.South San FranciscoUSA
  2. 2.European Molecular Biology LaboratoryHamburgGermany

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