Subtilisin Enzymes

Practical Protein Engineering

  • Richard Bott
  • Christian Betzel

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 379)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Structural Studies of Subtilisin Enzymes Alone and in Complex with Inhibitors

    1. Zbigniew Dauter, Christian Betzel, Nicolay Genov, Keith S. Wilson
      Pages 1-4
    2. Alexei Teplyakov, Piet Gros, Wim G. J. Hol
      Pages 5-9
    3. T. P. Singh, B. Padmanabhan, P. Narula, A. K. Saxena, C. Betzel, P. Sharma et al.
      Pages 11-20
    4. Sabine Pfeffer-Hennig, Zbigniew Dauter, Michael Hennig, Wolfgang Höhne, Keith Wilson, Christian Betzel
      Pages 29-41
    5. Christian Betzel, Silke Klupsch, Sven Branner, Keith S. Wilson
      Pages 49-61
  3. New Enzymes

  4. Structure-Function Studies

    1. Hanne Grøn, Lene M. Bech, Steen Bech Sørensen, Morten Meldal, Klaus Breddam
      Pages 105-112
    2. A. M. Wolff, M. S. Showell, M. G. Venegas, B. L. Barnett, W. C. Wertz
      Pages 113-120
    3. B. L. Barnett, Cathleen B. Turner
      Pages 121-131
  5. Biophysical Probes and Mutagenesis

    1. K. Peters, D. Brömine, G. Jahreis, S. Fittkau
      Pages 133-140
    2. Nicolay Genov, Peter Nikolov, Christian Betzel, Keith Wilson
      Pages 141-145
    3. Gary L. Gilliland, D. Travis Gallagher, Patrick Alexander, Philip Bryan
      Pages 159-169
    4. Alexander Müller, Wolfram Saenger
      Pages 183-189
  6. Protein Engineering od Subtilisin Enzymes

    1. J. M. van der Laan, O. Misset, L. J. S. M. Mulleners, G. Gerritse, H. N. Scheffers, D. J. van Schouwen et al.
      Pages 203-218
    2. M. R. Egmond, W. P. Antheunisse, P. Ravestein, A. T. A. Mooren, J. de Vlieg
      Pages 219-228
    3. Gudrun Lange, Christian Betzel, Keith Wilson, Sven Branner
      Pages 229-233
    4. Ole H. Olsen, Jan T. Pedersenn, Christian Betzel, Susanne Eschenburg, Sven Branner, Sven Hastrup
      Pages 235-241
    5. C. Paech, D. W. Goddette, T. Christianson, C. R. Wilson
      Pages 257-268
    6. Hiroshi Takagi, Hiroshi Matsuzawa, Takahisa Ohta, Makari Yamasaki, Masayori Inouye
      Pages 269-275
    7. Richard Bott, Judy Dauberman, Lori Wilson, Grant Ganshaw, Helena Sagar, Thomas Graycar et al.
      Pages 277-283
  7. Back Matter
    Pages 285-288

About this book


Subtilisin is the most extensively studied model system for protein engineering. The primary motivating factor for the interest in subtilisin is the commercial utility of this class of proteases. The subtilisin symposium was the first international meeting to bring together a large number of groups that have focused on the subtilisins and the subtilases-the protein superfamily of subtilisin-like enzymes. The results presented at the symposium are in this way a unique compendium of a broad spectrum of work largely focused on harnessing the potential of site-directed mutagenesis to understand and deliberately alter the function of these enzymes toward a desired end. This sort of protein engineering has been extremely successful in subtilisin, with many such "engineered" enzymes now widely used in commer­ cial enterprises. In this regard the experience derived from subtilisin does represent practical protein engineering. It is becoming clear that subtilisin represents a larger class of enzymes, the subtilases, that include many of the human pro hormone-converting enzymes. As international collabo­ rative efforts to sequence entire genomes continue, we can reasonably expect that additional members of the subtilase class will be encountered. Whenever interest in a member of this class of enzyme arises, the work on subtilisin will serve as a guide to the analysis for what in bacillus, fungi, and industry is an everyday workhorse enzyme.


enzyme enzymes fungi genes protein protein engineering system

Editors and affiliations

  • Richard Bott
    • 1
  • Christian Betzel
    • 2
  1. 1.Genecor International, Inc.South San FranciscoUSA
  2. 2.European Molecular Biology LaboratoryHamburgGermany

Bibliographic information

  • DOI
  • Copyright Information Springer-Verlag US 1996
  • Publisher Name Springer, Boston, MA
  • eBook Packages Springer Book Archive
  • Print ISBN 978-0-306-45108-9
  • Online ISBN 978-1-4613-0319-0
  • Series Print ISSN 0065-2598
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals