Micromanipulation by Light in Biology and Medicine

The Laser Microbeam and Optical Tweezers

  • Karl Otto Greulich

Part of the Methods in Bioengineering book series (MB)

Table of contents

  1. Front Matter
    Pages I-XII
  2. Karl Otto Greulich
    Pages 7-51
  3. Karl Otto Greulich
    Pages 53-96
  4. Karl Otto Greulich
    Pages 159-203
  5. Karl Otto Greulich
    Pages 205-250
  6. Karl Otto Greulich
    Pages 251-295
  7. Back Matter
    Pages 297-300

About this book


There are probably few people who do not dream of the good old times, when do­ ing science often meant fascination, excitement, even adventure. In our time, do­ ing science involves often technology and, perhaps, even business. But there are still niches where curiosity and fascination have their place. The subject of this book, technological as its title may sound, is one of the fortunate examples. It will report on lasers generating the coldest places in the Universe, and on table top laser microtools which can produce a heat "inferno" as it prevails in the interior of the Sun, or simulate, for specific plant cells, microgravity of the space around our plan­ et Earth. There will be some real surprises for the reader. The applications range from basic studies of the driving forces of cell division (and thus life) via genetic modification of cells (for example, for plant breeding) to medical applications such as blood cell analysis and finally in vitro fertilization. What are these instruments: laser microbeams and optical tweezers? Both are lasers coupled with a fluorescence microscope. The laser microbeam uses a pulsed ultraviolet laser. Light is focused, as well as possible, in space and time, in order to obtain extremely high light intensities - high enough to generate, for a very short instant, extremely hot spots which can be used to cut, fuse or perforate biological material.


Activation Micromanipulation Optical Tweezers Viruses blood cell cell biology cell division cells electromagnetic imaging mechanics microscopy proteins surgery tissue

Authors and affiliations

  • Karl Otto Greulich
    • 1
  1. 1.Institut für Molekulare Biotechnologie e.V.JenaGermany

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