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Techniques in Quantification and Localization of Gene Expression

  • Bruce K. Patterson

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Basic Methods

    1. Front Matter
      Pages 1-1
    2. Charles L. Goolsby, Cathy James
      Pages 3-22
    3. Bruce K. Patterson, Daniel K. Jiyamapa
      Pages 23-34
    4. Bruce K. Patterson
      Pages 45-53
    5. Ulf Andersson, Mark J. Litton, Tom E. Fehniger, Ann-Kristin Ulfgren, Jan Andersson
      Pages 55-79
    6. Vincent P. Antao, Audrey N. Player, Janice A. Kolberg
      Pages 81-93
  3. Specific Applications

  4. Back Matter
    Pages 147-150

About this book

Introduction

Can the son or daughter of a baseball pitcher or cricket bowler throw a ball 100 miles an hour? Is the son or daughter of an opera singer also an opera singer? Is a house with functional light switches lit? The line of thinking in these rhetorical questions also applies to human genetics. What do baseball pitchers, opera sing­ ers, light switches, and the Human Genome Project have in common? These questions address the issue of potential versus realization of function. Although sons and daughters of baseball pitchers and opera singers may have inherited the mechanical attributes to be baseball pitchers and opera singers, they may not, at any point in time, be baseball pitchers or opera singers. A house with functional light switches is not lit unless the light switches are on. Similarly, all of the genes discovered and sequenced as a result of the Human Genome Project are not expressed at the same time. Genome project information will allow us to deter­ mine the repertoire of genes in an individual, which is analogous to determining where the light switches in a house are located and whether they are functional (a mutation or deletion in the Genome Project Model). The pattern of "on" light switches in a house gives us functional information as to what the family inside is doing (e. g. , eating, reading, sleeping). Similarly, the pattern of gene expression (RNA) gives us information on what our bodies are doing (e. g.

Keywords

DNA HIV RNA biotechnology cell lines cytokine fluorescence gene expression genes genetics hybridization molecular biology protein tissue virus

Editors and affiliations

  • Bruce K. Patterson
    • 1
  1. 1.Laboratory of Viral Pathogenesis, Department of Pediatrics, Division of Special Infectious DiseasesChildren’s Memorial HospitalChicagoUSA

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