© 2011

Membrane Potential Imaging in the Nervous System

Methods and Applications

  • Marco Canepari
  • Dejan Zecevic

Table of contents

  1. Front Matter
    Pages i-x
  2. Marco Canepari, Marko Popovic, Kaspar Vogt, Knut Holthoff, Arthur Konnerth, Brian M. Salzberg et al.
    Pages 25-41
  3. Marco Canepari, Peter Saggau, Dejan Zecevic
    Pages 43-52
  4. William N. Frost, Jean Wang, Christopher J. Brandon, Caroline Moore-Kochlacs, Terrence J. Sejnowski, Evan S. Hill
    Pages 53-60
  5. Kevin L Briggman, William B. Kristan, Jesús E. González, David Kleinfeld, Roger Y. Tsien
    Pages 61-70
  6. Xiaoying Huang, Weifeng Xu, Kentaroh Takagaki, Jian-Young Wu
    Pages 71-81
  7. Amiram Grinvald, David Omer, Shmuel Naaman, Dahlia Sharon
    Pages 97-111
  8. Gaddum Duemani Reddy, Peter Saggau
    Pages 139-145
  9. Leslie M. Loew, Aaron Lewis
    Pages 147-155
  10. Lei Jin, Hiroki Mutoh, Thomas Knopfel, Lawrence B. Cohen, Thom Hughes, Vincent A. Pieribone et al.
    Pages 157-163
  11. Back Matter
    Pages 165-168

About this book


Membrane Potential Imaging in the Nervous System: Methods and Applications describes optical techniques to monitor neuronal membrane potential signals. The knowledge upon which voltage-imaging technique is based was generated over several decades and the aim of this book is to provide a single comprehensive source of information on different types of voltage-imaging techniques, including overviews, methodological details, examples of experimental measurements, and future developments. The book is structured in five sections, each containing several chapters written by experts and major contributors to particular topics. The volume starts with a historical perspective and fundamental principles of membrane potential imaging and continues to cover the measurement of membrane potential signals from dendrites and axons of individual neurons, measurements of the activity of many neurons with single cell resolution, monitoring of population signals from the nervous system, and concludes with the overview of new approaches to voltage-imaging. Membrane Potential Imaging in the Nervous System is targeted at all scientists interested in this mature but also rapidly expanding imaging approach.

Editors and affiliations

  • Marco Canepari
    • 1
  • Dejan Zecevic
    • 2
  1. 1.Université Joseph Fourier, Unité Inserm 836Grenoble Institute of NeuroscienceGrenobleFrance
  2. 2.Dept. Cellular &, Molecular PhysiologyYale University School of MedicineNew HavenUSA

About the editors

Dejan Zecevic (b. Belgrade 1948) is a Research Scientist at the Department of Cellular and Molecular Physiology, Yale University School of Medicine. He received the PhD in Biophysics from The University of Belgrade, Serbia and was trained in the laboratory of Dr Lawrence Cohen who initiated the field of voltage-sensitive dye recording. Dejan is the pioneer of intracellular voltage-sensitive dye imaging technique, a unique and a cutting edge technology for monitoring the membrane potential fluctuation in dendritic spines and fine branches. Marco (b. Milan 1970) is first class INSERM researcher (CR1) working at the Grenoble Institute of Neuroscience. He graduated in physics at the University of Genoa and received his PhD in biophysics from the International School for Advanced Studies in Trieste. He worked at the National Institute for Medical Research in London, at Yale University and at the University of Basel. Marco is expert on several optical techniques applied to neurophysiology. Marco and Dejan collaborated for a number of years using voltage-imaging and calcium imaging approaches to study mechanisms underlying synaptic plasticity.

Bibliographic information

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“This is an excellent ‘how to’ and methods approach to single, multiple, and populational photon imaging using various dyes optically in what is now recognized as a cutting edge technique. … This is a very technical book for intermediate to advanced neuroscientists and would be useful for both the office and laboratory. … I highly recommend this book for all interested audiences.” (Joseph J. Grenier,, April, 2015)