Practical Manual of In Vitro Fertilization

Advanced Methods and Novel Devices

  • Zsolt Peter Nagy
  • Alex C. Varghese
  • Ashok Agarwal

Table of contents

  1. Front Matter
    Pages i-xxiii
  2. Setting Up and Running an IVF Laboratory

    1. Front Matter
      Pages 7-7
    2. Dean E. Morbeck, Marlena Duke
      Pages 9-15
    3. Johan Guns, Ronny Janssens, Martine Vercammen
      Pages 17-25
    4. Nancy L. Bossert, Christopher De Jonge
      Pages 27-32
    5. William R. Boone, H. Lee Higdon III
      Pages 33-39
    6. Michael A. Britt, Klaus E. Wiemer
      Pages 41-44
    7. Peter Sjoblom, Julius Hreinsson
      Pages 45-51
  3. IVF Laboratory Equipment and Culture Systems

    1. Front Matter
      Pages 53-53
    2. Leslie Weikert, Christa Fralick, Klaus E. Wiemer
      Pages 55-59
    3. Marius Meintjes
      Pages 61-70
    4. Nicolas Prados, Alex C. Varghese
      Pages 71-78
    5. Jason E. Swain, Thomas B. Pool
      Pages 79-90
  4. In Vitro Fertilization

    1. Front Matter
      Pages 91-91
    2. Roberta Maggiulli, Filippo Ubaldi, Laura Rienzi
      Pages 93-104
    3. Basak Balaban, Turgay Barut, Bulent Urman
      Pages 105-119
    4. Markus Montag, Maria Köster, Hans van der Ven
      Pages 121-125
  5. Embryo Culture Methods

    1. Front Matter
      Pages 131-131
    2. David K. Gardner, Michelle Lane
      Pages 141-150
    3. Baris Ata, Jack Huang, Ri-Cheng Chian
      Pages 151-160
    4. Claude Ranoux
      Pages 161-169
    5. Gary D. Smith, Charles Bormann, Shuichi Takayama
      Pages 171-181
  6. Sperm Processing and Selection

    1. Front Matter
      Pages 183-183
    2. Margot Flint, Fanuel Lampiao, Ashok Agarwal, Stefan S. du Plessis
      Pages 185-192
    3. De Yi Liu, Harold Bourne, Claire Garrett, Gary N. Clarke, Shlomi Barak, H. W. Gordon Baker
      Pages 193-198
    4. Ralf Henkel
      Pages 199-205
    5. Sandro C. Esteves, Sidney Verza Jr.
      Pages 207-220
    6. Thamara Viloria, Marcos Meseguer, Antonio Pellicer, José Remohí, Nicolás Garrido
      Pages 221-228
    7. P. Vanderzwalmen, Magnus Bach, Batsuren Baramsai, A. Neyer, Delf Schwerda, Astrid Stecher et al.
      Pages 229-240
    8. Steven Fleming, John Aitken
      Pages 259-263
    9. Enver Kerem Dirican
      Pages 265-272
    10. Luca Gianaroli, Cristina Magli, Andor Crippa, Giorgio Cavallini, Eleonora Borghi, Anna P. Ferraretti
      Pages 273-277
  7. Insemination Procedures

    1. Front Matter
      Pages 279-279
    2. Gautam N. Allahbadia, Rubina Merchant
      Pages 281-296
    3. Liesl Nel-Themaat, Thomas Elliott, Ching-Chien Chang, Graham Wright, Zsolt Peter Nagy
      Pages 297-305
    4. Gianpiero D. Palermo, Queenie V. Neri, Devin Monahan, Takumi Takeuchi, Peter N. Schlegel, Zev Rosenwaks
      Pages 307-320
  8. Micromanipulators and Micromanipulation

    1. Front Matter
      Pages 327-327
    2. Hubert Joris
      Pages 329-334
    3. Steven Fleming, Catherine Pretty
      Pages 335-340
    4. Christiane Wittemer, Bruno Laborde, Frederic Ribay, Stephane Viville
      Pages 347-352
    5. Zhe Lu, Xinyu Liu, Xuping Zhang, Clement Leung, Navid Esfandiari, Robert F. Casper et al.
      Pages 353-359
    6. Akira Onishi, Anthony C. F. Perry
      Pages 371-375
  9. Embryo Evaluation, Grading, and Assisted Hatching

    1. Front Matter
      Pages 377-377

About this book


The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of references. The result is a readily accessible, high quality reference guide for reproductive endocrinologists, urologists, embryologists, biologists and research scientists. The Manual also offers an excellent description of novel procedures that will likely be employed in the near future.  An indispensable resource for physicians and basic scientists, the Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is an invaluable reference and addition to the literature.


ART unit Assisted hatching Azoospermia Biopsy procedure Cryopreservation Culture Embryo Embryo evaluation Embryo transfer Gametes IVF lab In vitro fertilization Insemination Oocyte Ovum Sperm assessment Sperm processing Sperm selection Testicular sperm extraction Ultrasound

Editors and affiliations

  • Zsolt Peter Nagy
    • 1
  • Alex C. Varghese
    • 2
  • Ashok Agarwal
    • 3
  1. 1., Scientific and Laboratory DirectorReproductive Biology AssociatesAtlantaUSA
  2. 2., Sr. EmbryologistMontreal Reproductive CentreMontrealCanada
  3. 3.Center for Reproductive Medicine, Department of UrologyCleveland ClinicClevelandUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Springer, New York, NY
  • eBook Packages Medicine Medicine (R0)
  • Print ISBN 978-1-4419-1779-9
  • Online ISBN 978-1-4419-1780-5
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals
Internal Medicine & Dermatology