© 2010

Steroid Analysis

  • Hugh L. J. Makin
  • D.B. Gower

Table of contents

  1. Front Matter
    Pages I-XXII
  2. Alexander Kasal
    Pages 1-25
  3. Alexander Kasal, Milos Budesinsky, William J. Griffiths
    Pages 27-161
  4. Hugh L. J. Makin, John W. Honour, Cedric H. L. Shackleton, William J. Griffiths
    Pages 163-282
  5. M. J. Wheeler, G. Barnard
    Pages 283-327
  6. Robert Fraser, D. B. Gower, John W. Honour, Mary C. Ingram, Andrew T. Kicman, Hugh L. J. Makin et al.
    Pages 329-455
  7. P. J. Wood, D. B. Gower
    Pages 559-603
  8. Geoff Holder, Hugh L. J. Makin, H. Leon Bradlow
    Pages 605-742
  9. A. T. Kicman, E. Houghton, D. B. Gower
    Pages 743-836
  10. Jan Sjövall, William J. Griffiths, Kenneth D. R. Setchell, Nariyasu Mano, Junichi Goto
    Pages 837-966
  11. Hugh L. J. Makin, Glenville Jones, Martin Kaufmann, Martin J. Calverley
    Pages 967-1096
  12. Sándor Görög
    Pages 1097-1153
  13. Jonathan G. Middle
    Pages 1155-1188
  14. Back Matter
    Pages 1189-1224

About this book


This comprehensive and up-to-date 2nd edition of the Steroid Analysis handbook, written by a team of distinguished experts from around the world concentrates on the analysis of steroids in biological fluids. This edition is based on the same format as the first, primarily dealing with analysis of low levels of steroid analytes in biological fluids, but including an extra chapter on pharmaceutical aspects of steroid analysis. Spectroscopic and other methods, including UV and IR absorption spectroscopy, NMR spectroscopy, mass spectrometry, X-ray diffraction, chromatography and immunoassay of steroids are treated in detail. Discussion of general methods for extraction, purification and quantitation is followed by consideration of the analysis of specific important steroid groups, such as progestagens, estrogens, corticosteroids, vitamins D, bile acids and anabolic steroids and their metabolites. The editors have made a conscious decision to leave the first edition to stand on its own with regards to steroid analysis prior to 1995 and authors therefore were asked to review the modern post-1995 steroid scene, though pre-1995 data were not excluded if authors felt it was necessary. This book should be of interest to analytical chemists, biochemists and chemical pathologists with an interest or involvement in steroids.


analysis of androgens analysis of bile acids analysis of corticosteroids analysis of estrogens analysis of progestagens analysis of vitamin D analytes in complex matrices biological fluids detection of anabolic steroids in sport doping and detect

Editors and affiliations

  • Hugh L. J. Makin
    • 1
  • D.B. Gower
    • 2
  1. 1.Barts & the London School of, Medicine & DentistryQueen Mary University of LondonLondonUnited Kingdom
  2. 2.Orpington, KentUnited Kingdom

About the editors

Hugh Makin obtained his PhD at the institute of Child Health, University of London in 1966 and was awarded the degree DSc in 1996 for his research on steroid analysis.

He has been successively Lecturer, Senior Lecturer and Professor in the Department of Clinical Biochemistry at St. Bartholomew's & the Royal London School of Medicine & Dentistry since joining the Medical School in 1967. His research interests involve the use of for the measurement of vitamin D metabolites and, in collaboration with Glen Jones, the use of mass spectrometry for the identification of calcitriol analogue metabolites both in vivo and in vitro.

Hugh was the Programme Adviser to the UK Food Standards Agency Phytoestrogen Research Programme which supported a phytoestrogen quality assurance scheme organised by the Institute of Food Research in Norwich.

D.B.Gower is Emeritus Professor of Steroid Biochemistry in the University of London, United Kingdom. He holds a B.Sc. in Chemistry(1955) and a Ph.D. in Biochemistry(1959) from the University of London and in 1972 he was awarded a D.Sc (Lond.) for his research into the biochemical mechanisms for the control of steroid hormone formation and the pheromonal properties of some C19 steroids.

His University teaching and research career began in 1960 at Guy's Hospital Medical School, London and in 1986, he was promoted to Professor and Vice-Chairman of the Division of Biochemistry in the United Medical and Dental Schools of Guy's and St. Thomas's (now merged with King's College London).

Professor Gower is a Chartered Chemist and Fellow of the Royal Society of Chemistry as well as a Chartered Biologist and Fellow of The Institute of Biology.

Bibliographic information

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From the reviews of the second edition:

This is an excellent, comprehensive book and can be highly recommended to those who want an up-to-date reference on steroid analysis

“A comprehensive compendium of the broad and complex topics of characterizing and measuring steroidal compounds. … The book is composed of 13 chapters written by 26 experts. … Comprehensiveness and timeliness are particularly unique features of this book. … an interesting and highly recommended read. … a unique book has been produced covering analytical aspects of steroid biochemistry, detection , and quantification in their entire breadth. The clear and balanced content is a well structured source of information for students and researchers in steroid analysis.” (Mario Thevis, Analytical Bioanalytical Chemistry, Vol. 401, 2011)