© 2007

Protocols for Micropropagation of Woody Trees and Fruits

  • S. Mohan Jain
  • H. Häggman

Table of contents

  1. Front Matter
    Pages i-x
  2. Section A

    1. Front Matter
      Pages 2-2
    2. P. B. Gahan
      Pages 3-14
    3. W. Tang, R. J. Newton
      Pages 15-22
    4. S. S. Korban, I. -W. Sul
      Pages 23-32
    5. R. J. Ordás, P. Alonso, C. Cuesta, M. Cortizo, A. Rodríguez, B. Fernández
      Pages 33-39
    6. K. Ishii, Y. Hosoi, E. Maruyama
      Pages 41-50
    7. C. Hargreaves, M. Menzies
      Pages 51-65
    8. C. Santos, J. Loureiro, T. Lopes, G. Pinto
      Pages 67-83
    9. M. G. Ostrolucká, A. Gajdošová, G. Libiaková
      Pages 85-91
    10. D. T. Nhut, N. T. T. Hien, N. T. Don, D. V. Khiem
      Pages 107-116
    11. R. Rodríguez, L. Valledor, P. Sánchez, M. F. Fraga, M. Berdasco, R. Hasbún et al.
      Pages 137-146
    12. J. W. Van Sambeek, J. E. Preece
      Pages 179-192
    13. J. Zhang, Y. Liu, H. Wang
      Pages 193-199

About this book


Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.


Embryo Flora Fruits Large-scale plant multiplication Micropropagation Neem Papaya Woody Trees Woody plant forest fruit woody plants woody species

Editors and affiliations

  • S. Mohan Jain
    • 1
  • H. Häggman
    • 2
  1. 1.University of HelsinkiFinland
  2. 2.University of OuluFinland

Bibliographic information