Reviews and Protocols in DT40 Research

Subcellular Biochemistry

  • Jean-Marie Buerstedde
  • Shunichi Takeda

Part of the Subcellular Biochemistry book series (SCBI, volume 40)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Jean-Marie Buerstedde, Hiroshi Arakawa
    Pages 11-24
  3. Randolph B. Caldwell, Andrzej M. Kierzek
    Pages 25-37
  4. William R. A. Brown, Margaret C. M. Smith, Felix Dafhnis-Calas, Sunir Malla, Zhengyao Xu
    Pages 39-48
  5. Masayuki Seki, Shusuke Tada, Takemi Enomoto
    Pages 49-73
  6. Sandrine Ruchaud, Kumiko Samejima, Damien Hudson, Scott H. Kaufmann, William C. Earnshaw
    Pages 75-90
  7. Masahiro Okada, Tetsuya Hori, Tatsuo Fukagawa
    Pages 91-106
  8. Michael D. Rainey, George Zachos, David A. F. Gillespie
    Pages 107-117
  9. Frank R. Wettey, Antony P. Jackson
    Pages 119-143
  10. Hisaaki Shinohara, Tomohiro Kurosaki
    Pages 145-187
  11. Jukka Alinikula, Olli Lassila, Kalle-Pekka Nera
    Pages 189-205
  12. Hidehiko Kikuchi, Hirak Kumar Barman, Masami Nakayama, Yasunari Takami, Tatsuo Nakayama
    Pages 225-243
  13. Paul E. Neima, Joan Burnside, Katrina Elsaesser, Harry Hwang, Bruce E. Clurman, Robert Kimmel et al.
    Pages 245-256
  14. Anne-Laure Perraud, Carsten Schmitz, Andrew M. Scharenberg
    Pages 257-270
  15. Julian E. Sale, Anna-Laura Ross, Laura J. Simpson
    Pages 271-294
  16. Minoru Takata, Kazuhiko Yamamoto, Nobuko Matsushita, Hiroyoki Kitao, Sciki Hirano, Masamichi Ishaiai
    Pages 295-311
  17. Helfrid Hochegger, Shunichu Takeda
    Pages 313-325
  18. Ken-ichi Yamamoto, Masahiko Kobayashi, Hiroko Shimizu
    Pages 327-339
  19. Julian E. Sale
    Pages 341-344
  20. Huseyin Saribasak, Hiroshi Arakawa
    Pages 345-346
  21. Hiroshi Arakawa
    Pages 353-354
  22. David A. F. Gillespie, Mark Walker
    Pages 355-358
  23. David A. F. Gillespie, Catarina Henriques
    Pages 359-361
  24. Robert Kimmel, Amalia Icreverzi, Paul Neiman
    Pages 363-371
  25. Anne-Laure Perraud, Carsten Schmitz, Andrew M. Scharenberg
    Pages 373-378
  26. Roger Franklin, Julian E. Sale
    Pages 379-382
  27. Felix Randow, Julian E. Sale
    Pages 383-386
  28. Laura. J. Simpson, Julian E. Sale
    Pages 387-391
  29. Jean-Marie Buerstedde
    Pages 393-394
  30. Dávid Szüts, Julian E. Sale
    Pages 395-398
  31. Laura J. Simpson, Julian E. Sale
    Pages 399-403
  32. Roger Franklin, Julian E. Sale
    Pages 405-408
  33. Eiichiro Sonoda
    Pages 415-418
  34. Huseyin Saribasak, Hiroshi Arakawa
    Pages 419-421
  35. Frank R. Wettey, Antony P. Jackson
    Pages 423-425
  36. Frank R. Wettey, Antony P. Jackson
    Pages 427-429
  37. Frank R. Wettey, Antony P. Jackson
    Pages 431-434
  38. Yan-Dong Wang, Randolph B. Caldwell
    Pages 439-440
  39. Back Matter
    Pages 441-477

About this book


Research is fast paced and advances in RNA interference have recently opened up new opportunities for genetic experiments in human cell lines. However, the possibility to easily modify the genome still remains a powerful tool to investigate the function of coding and regulatory sequences in the vertebrate genome. DT40 has never been a quick and easy road to fame. It would be unfair to blame this on DT40 as it has proven to be a reliable and robust companion with fast doubling time, easy clonability and a relatively stable karyotype. If this model system is going to flourish over the next 15 years, it will be thanks to ingenious and original researchers. They may feel as if they work outside the mainstream, but they can take heart by the fact that only the clever exploitation of diversity and conservation makes biological research both elegant and rewarding. It is with this in mind that the DT40 handbook has been conceived and written. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been kindly provided by a number of laboratories will be particularly useful in this regard.


Calcium Chaperone DNA Endoplasmatisches Reticulum Microarray PCR RNA Termination biochemistry cell lines enzymes gene expression hybridization regulation transcription

Editors and affiliations

  • Jean-Marie Buerstedde
    • 1
  • Shunichi Takeda
    • 2
  1. 1.GSF National Research CentreNeuherbergGermany
  2. 2.Kyoto UniversityJapan

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