© 2020

Virus-Induced Gene Silencing in Plants

Methods and Protocols

  • Vincent Courdavault
  • Sébastien Besseau

Part of the Methods in Molecular Biology book series (MIMB, volume 2172)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Keisuke Tasaki, Masumi Yamagishi, Chikara Masuta
    Pages 1-13
  3. Ying-Wen Huang, Chao-Yuan Chang, Yau-Heiu Hsu
    Pages 15-25
  4. Magda Lisette Arce-Rodríguez, Neftalí Ochoa-Alejo
    Pages 27-38
  5. Jinping Zhao, Haolang Jiang, Guanyu Wang, Zonghua Wang, Jingao Dong, Junqi Song
    Pages 39-50
  6. Syed Shan-e-Ali Zaidi, Kumar Vasudevan, Ezequiel Matias Lentz, Hervé Vanderschuren
    Pages 51-64
  7. Rongji Chen, Xue Chen, Jillian M. Hagel, Peter J. Facchini
    Pages 75-92
  8. Pamela Lemos Cruz, María Isabel Restrepo, Thomas Dugé de Bernonville, Audrey Oudin, Thibaut Munsch, Arnaud Lanoue et al.
    Pages 93-110
  9. Lira Palmer, Sarah E. O’Connor
    Pages 111-121
  10. Rajesh Chandra Misra, Shubha Sharma, Anchal Garg, Sumit Ghosh
    Pages 123-138
  11. Dikki Pedenla Bomzan, H. B. Shilpashree, P. Anjali, Sarma Rajeev Kumar, Dinesh A. Nagegowda
    Pages 139-154
  12. Konstantinos Koudounas, Margarita Thomopoulou, Elisavet Angeli, Dikran Tsitsekian, Stamatis Rigas, Polydefkis Hatzopoulos
    Pages 165-182
  13. Carolina Werner Ribeiro, Thomas Dugé de Bernonville, Gaëlle Glévarec, Arnaud Lanoue, Audrey Oudin, Olivier Pichon et al.
    Pages 183-197
  14. Shaun R. Broderick, Laura J. Chapin, Michelle L. Jones
    Pages 199-222
  15. Huijun Yan, Zhao Zhang, Jean-Louis Magnard, Benoît Boachon, Sylvie Baudino, Kaixue Tang
    Pages 223-232
  16. Jinping Zhao, Guanyu Wang, Haolang Jiang, Tingli Liu, Jingao Dong, Zonghua Wang et al.
    Pages 243-257
  17. Back Matter
    Pages 259-261

About this book


This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field.


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Editors and affiliations

  • Vincent Courdavault
    • 1
  • Sébastien Besseau
    • 2
  1. 1.EA2106 Biomolécules et Biotechnologies VégétalesUniversité de ToursToursFrance
  2. 2.EA2106 Biomolécules et Biotechnologies VégétalesUniversité de ToursToursFrance

Bibliographic information

  • Book Title Virus-Induced Gene Silencing in Plants
  • Book Subtitle Methods and Protocols
  • Editors Vincent Courdavault
    Sébastien Besseau
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0750-3
  • Softcover ISBN 978-1-0716-0753-4
  • eBook ISBN 978-1-0716-0751-0
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XII, 261
  • Number of Illustrations 10 b/w illustrations, 48 illustrations in colour
  • Topics Plant Sciences
    Plant Genetics and Genomics
  • Buy this book on publisher's site
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