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© 2020

Caveolae

Methods and Protocols

  • Cedric M. Blouin
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2169)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Stine K. Morthorst, Johanne B. Mogensen, Søren T. Christensen, Lotte B. Pedersen
    Pages 27-41
  3. Tomoya Yamaguchi, Toyoshi Fujimoto, Takashi Takahashi
    Pages 43-52
  4. Adriana M. Zimnicka, Zhenlong Chen, Peter T. Toth, Richard D. Minshall
    Pages 71-80
  5. Joanna Podkalicka, Cedric M. Blouin
    Pages 81-88
  6. Elin Larsson, Madlen Hubert, Richard Lundmark
    Pages 119-127
  7. Sarah M. Plucinsky, Jeffrey A. Julien, Kerney Jebrell Glover
    Pages 137-147
  8. Ye-Wheen Lim, Harriet P. Lo, Thomas E. Hall, Robert G. Parton
    Pages 175-187
  9. Back Matter
    Pages 217-218

About this book

Introduction

This volume explores techniques used to study either the structure or the functions of caveolae and their components in several normal and pathophysiological situations. The chapters in this book cover topics such as selective visualization of caveolae by electron microscopy techniques; spatiotemporal analysis of caveolae dynamics and mechanics using live cell fluorescence microscopy; in vitro reconstitution such as liposomes and GPMVs as tools to study caveolin- and cavin-interacting partners; pulling of tethers from the cell plasma membrane using optical tweezers; and immunofluorescence-based analysis of caveolin-3 in the diagnostic management of neuromuscular diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Caveolae: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and proteins composing their coat in various model organisms.  

Keywords

electron microscopy protein purification fluorescence microscopy spinning disk microscopy endothelial cells

Editors and affiliations

  • Cedric M. Blouin
    • 1
  1. 1.Institut Curie – Centre de Recherche, PSL Research UniversityMembrane Mechanics and Dynamics of Intracellular Signaling LaboratoryParisFrance

Bibliographic information

  • Book Title Caveolae
  • Book Subtitle Methods and Protocols
  • Editors Cedric M. Blouin
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI https://doi.org/10.1007/978-1-0716-0732-9
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0731-2
  • Softcover ISBN 978-1-0716-0734-3
  • eBook ISBN 978-1-0716-0732-9
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 218
  • Number of Illustrations 9 b/w illustrations, 37 illustrations in colour
  • Topics Lipidology
    Cell Biology
  • Buy this book on publisher's site
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