© 2020

Kupffer Cells

Methods and Protocols

  • Myriam Aouadi
  • Valerio Azzimato

Part of the Methods in Molecular Biology book series (MIMB, volume 2164)

Table of contents

  1. Front Matter
    Pages i-x
  2. Cecilia Morgantini
    Pages 11-13
  3. Emelie Barreby, Connie Xu
    Pages 27-44
  4. Qiushi Tang, Gwladys Gernoux, Yangfun Cheng, Terence Flotte, Christian Mueller
    Pages 75-85
  5. Camille Blériot, Shamin Li, Muhammad Faris Bin Mohd Kairi, Evan Newell, Florent Ginhoux
    Pages 87-99
  6. Mirko E. Minniti, Osman Ahmed, Matteo Pedrelli
    Pages 101-108
  7. Bader Zarrouki, Jeremie Boucher
    Pages 121-127
  8. Adeline Bertola
    Pages 145-157
  9. Back Matter
    Pages 159-160

About this book


This volume provides a complete overview of kupffer cell biology, from isolation to phenotyping. Chapters guide readers through methods and protocols on phenotyping, including RNA sequencing, Mass cytometry and 3D microscopy, as well as gene manipulation in vivo, and disease models from fatty liver disease to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and cutting-edge, Kupffer Cells: Methods and Protocols  aims to ensure successful investigation of this growing field. 


steato-hepatitis Mass Cytometry electron microscopy immunofluorescence DNA sequencing

Editors and affiliations

  • Myriam Aouadi
    • 1
  • Valerio Azzimato
    • 2
  1. 1.Department of Medicine, Integrated Cardio Metabolic CenterKarolinska InstitutetHuddingeSweden
  2. 2.Department of Medicine, Integrated Cardio Metabolic CenterKarolinska InstitutetHuddingeSweden

Bibliographic information

  • Book Title Kupffer Cells
  • Book Subtitle Methods and Protocols
  • Editors Myriam Aouadi
    Valerio Azzimato
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0703-9
  • Softcover ISBN 978-1-0716-0706-0
  • eBook ISBN 978-1-0716-0704-6
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages X, 160
  • Number of Illustrations 10 b/w illustrations, 24 illustrations in colour
  • Topics Cell Biology
  • Buy this book on publisher's site
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