Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2160)
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Table of contents (22 protocols)
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Back Matter
Keywords
About this book
This volume explores a collection of experimental techniques used to investigate different aspects of pollen development and function, including its role in reaching the ovule and delivering the two sperm cells. The techniques discussed range from basic methodology to cultivate pollen in vitro to the sophisticated experiments involving micromanipulation, Lab-on-Chip technology, or high-end imaging. The chapters in this book cover topics such as pollen grain counting using a cell counter; restricted pollination for tracing individual pollen tubes in a pistil; obtaining mutant pollen for phenotypic analysis and pollen tube dual-staining; analyzing intracellular gradients in pollen tubes; and measuring exocytosis rate in Arabidopsis pollen tube using corrected fluorescence recovery after photoconversion (cFRAPc) technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Pollen and Pollen Tube Biology: Methods and Protocols is a valuable resource for novice and expert scientists interested in learning more about the field of plant reproduction.
Editors and Affiliations
Bibliographic Information
Book Title: Pollen and Pollen Tube Biology
Book Subtitle: Methods and Protocols
Editors: Anja Geitmann
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0672-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0671-1Published: 12 June 2020
Softcover ISBN: 978-1-0716-0674-2Published: 12 June 2021
eBook ISBN: 978-1-0716-0672-8Published: 11 June 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 332
Number of Illustrations: 7 b/w illustrations, 90 illustrations in colour
Topics: Plant Sciences
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma