© 2020

Molecular Dermatology

Methods and Protocols

  • ​Natalia V. Botchkareva
  • Gillian E. Westgate

Part of the Methods in Molecular Biology book series (MIMB, volume 2154)

Table of contents

  1. Front Matter
    Pages i-x
  2. Richard Baker, M. Julie Thornton
    Pages 23-32
  3. Hanna Siiskonen, Jörg Scheffel
    Pages 33-43
  4. Isabelle Petit, Ayelet Levy, Daniel Aberdam
    Pages 63-72
  5. Ekaterina P. Kalabusheva, Ekaterina A. Vorotelyak
    Pages 153-163
  6. Mara Mancini, Anna Maria Lena, Eleonora Candi
    Pages 175-185
  7. Maximilian E. Pickup, Mohammed I. Ahmed
    Pages 187-196
  8. Carmit Bar, V. Julian Valdes, Elena Ezhkova
    Pages 197-215
  9. Andrei N. Mardaryev, Michael Y. Fessing
    Pages 217-230

About this book


This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situin vitroex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.


Hair follicles Gene expression Non-coding transcriptome Investigative dermatology Cell culture

Editors and affiliations

  • ​Natalia V. Botchkareva
    • 1
  • Gillian E. Westgate
    • 2
  1. 1.Monasterium Laboratory Skin and Hair Research Solutions GmbHMünsterGermany
  2. 2.Centre for Skin SciencesUniversity of BradfordBradfordUK

Bibliographic information

  • Book Title Molecular Dermatology
  • Book Subtitle Methods and Protocols
  • Editors ​Natalia V. Botchkareva
    Gillian E. Westgate
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0647-6
  • Softcover ISBN 978-1-0716-0650-6
  • eBook ISBN 978-1-0716-0648-3
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages X, 278
  • Number of Illustrations 6 b/w illustrations, 69 illustrations in colour
  • Topics Biochemistry, general
    Cell Biology
  • Buy this book on publisher's site
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“It is intended for researchers working in the areas of fundamental as well as applied dermatological science. In my opinion, this book is valuable for students, researchers, and dermatologists interested in learning about the methods and protocols in molecular dermatology. … This book is an essential resource for all students, researchers, and dermatologists interested in learning more about methods and protocols in molecular dermatology.” (Omer Iqbal, Doody's Book Reviews, October 30, 2020)