© 2020

In Situ Hybridization Protocols

  • Boye Schnack Nielsen
  • Julia Jones

Part of the Methods in Molecular Biology book series (MIMB, volume 2148)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. General Applications

  3. Methods for DNA ISH

    1. Front Matter
      Pages 33-33
    2. Sheila J. M. O’Connor, Kathryn R. Turner, Sharon L. Barrans
      Pages 35-70
    3. Guilherme T. Braz, Fan Yu, Lívia do Vale Martins, Jiming Jiang
      Pages 71-83
    4. Alasdair Jubb, Shelagh Boyle
      Pages 85-95
  4. Methods for Cultured Cells

    1. Front Matter
      Pages 97-97
    2. Jaslin P. James, Laura Johnsen, Trine Møller, Boye Schnack Nielsen
      Pages 99-110
  5. Methods for Wholemounts and Plant Material

    1. Front Matter
      Pages 157-157
    2. Toby G. R. Andrews, Giacomo Gattoni, Lara Busby, Michael A. Schwimmer, Èlia Benito-Gutiérrez
      Pages 179-194
    3. Theresa Gross-Thebing
      Pages 195-202
    4. Samar Sheat, Stephan Winter, Paolo Margaria
      Pages 203-215
  6. Automated Methods for RNA

    1. Front Matter
      Pages 217-217

About this book


This fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by researchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multiplexing combinations are explored. Chapters in this book are organized into seven sections and cover general applications; methods for DNA ISH; methods for cultured cells; methods for wholemount and plant material; automated methods for RNA; multiplexing and combined methods; and targeted selective methods and single molecule detections. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, In Situ Hybridization Protocols, Fifth Edition is a valuable resource for both novice and expert scientists interested in learning more about this exciting and advancing field. 


RNAscope Stellaris Fluidigm Nanostring circRNA

Editors and affiliations

  • Boye Schnack Nielsen
    • 1
  • Julia Jones
    • 2
  1. 1.Molecular HistologyBioneer A/SHorsholmDenmark
  2. 2.Cancer Research UKUniversity of CambridgeCambridgeUK

Bibliographic information

  • Book Title In Situ Hybridization Protocols
  • Editors Boye Schnack Nielsen
    Julia Jones
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0622-3
  • Softcover ISBN 978-1-0716-0625-4
  • eBook ISBN 978-1-0716-0623-0
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 5
  • Number of Pages XIII, 392
  • Number of Illustrations 1 b/w illustrations, 90 illustrations in colour
  • Topics Genetics and Genomics
  • Buy this book on publisher's site