© 2020

CRISPR-Cas Methods

  • M. Tofazzal Islam
  • Pankaj K. Bhowmik
  • Kutubuddin A. Molla

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Kutubuddin A. Molla, Subhasis Karmakar, M. Tofazzal Islam
    Pages 1-23
  3. Yvonne Ludwig, Inez H. Slamet-Loedin
    Pages 35-50
  4. Magdalena Śmiech, Paweł Leszczyński, Effi Haque, Hiroaki Taniguchi
    Pages 163-171
  5. Dinesh Adhikary, Isadora Louise Alves da Costa Ribeiro Quintans, Pankaj K. Bhowmik
    Pages 173-190
  6. Pankaj K. Bhowmik, M. Tofazzal Islam
    Pages 203-222
  7. Leena Tripathi, Valentine Otang Ntui, Jaindra Nath Tripathi
    Pages 223-236
  8. Dinesh Adhikary, Isadora Louise Alves da Costa Ribeiro Quintans, Pankaj K. Bhowmik
    Pages C1-C1
  9. Back Matter
    Pages 253-278

About this book


This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. 

Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.


Staphylococcus aureus Cas9 Brachypodium distachyon P. palmivora mutants deletion (knock-out) tagging (knock-in)

Editors and affiliations

  • M. Tofazzal Islam
    • 1
  • Pankaj K. Bhowmik
    • 2
  • Kutubuddin A. Molla
    • 3
  1. 1.Institute of Biotechnology and Genetic Engineering (IBGE)Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipurBangladesh
  2. 2.Department of Aquatic and Crop Resource DevelopmentNational Research Council of CanadaSASKATOONCanada
  3. 3.Crop Improvement DivisionICAR-National Rice Research InstituteCuttackIndia

About the editors

Bibliographic information

  • Book Title CRISPR-Cas Methods
  • Editors M. Tofazzal Islam
    Pankaj K. Bhowmik
    Kutubuddin A. Molla
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0615-5
  • Softcover ISBN 978-1-0716-0618-6
  • eBook ISBN 978-1-0716-0616-2
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages XIV, 278
  • Number of Illustrations 9 b/w illustrations, 58 illustrations in colour
  • Topics Genetics and Genomics
  • Buy this book on publisher's site