© 2020

Corneal Regeneration

Methods and Protocols

  • Mark Ahearne


  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 2145)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Richard M. Nagymihaly, Morten C. Moe, Goran Petrovski
    Pages 1-15
  3. Kim Santerre, Isabelle Xu, Mathieu Thériault, Stéphanie Proulx
    Pages 17-27
  4. Marina López-Paniagua, Teresa Nieto-Miguel, Sara Galindo, Laura García-Posadas, Ana de la Mata, Rosa M. Corrales et al.
    Pages 39-49
  5. James W. Foster, Karl J. Wahlin, Shukti Chakravarti
    Pages 51-58
  6. Tara Moore, Connie Chao-Shern, Larry DeDionisio, Kathleen A. Christie, M. Andrew Nesbit
    Pages 59-75
  7. Liujiang Song, Jacquelyn J. Bower, Matthew L. Hirsch
    Pages 77-102
  8. Gaëtan Le-Bel, Pascale Desjardins, Camille Couture, Lucie Germain, Sylvain L. Guérin
    Pages 103-118
  9. Mehmet Gürdal, Gülinnaz Ercan, Dimitrios I. Zeugolis
    Pages 119-141
  10. Andrew Hopkinson, Emily R. Britchford, Laura E. Sidney
    Pages 143-157
  11. Mark Ahearne, Julia Fernández-Pérez
    Pages 159-168
  12. Elle Edin, Fiona Simpson, May Griffith
    Pages 169-183
  13. Robert Thomas Brady, Peter W. Madden
    Pages 215-230
  14. Keith M. Meek, Andrew J. Quantock, Sally Hayes, James Bell
    Pages 231-247
  15. Marina López-Paniagua, Teresa Nieto-Miguel, Sara Galindo, Laura García-Posadas, Ana de la Mata, Rosa M. Corrales et al.
    Pages C1-C1
  16. Back Matter
    Pages 249-250

About this book


This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, how to evaluate the success of these therapies, and cell and material characterization techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Corneal Regeneration: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.


limbal stem cell Immunomodulation collagen scaffolds corneal repair endothelial keratoplasty

Editors and affiliations

  • Mark Ahearne
    • 1
  1. 1.Trinity Centre for Biomedical EngineeringTrinity College DublinDublinIreland

Bibliographic information

  • Book Title Corneal Regeneration
  • Book Subtitle Methods and Protocols
  • Editors Mark Ahearne
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0598-1
  • Softcover ISBN 978-1-0716-0601-8
  • eBook ISBN 978-1-0716-0599-8
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XII, 250
  • Number of Illustrations 23 b/w illustrations, 43 illustrations in colour
  • Topics Regenerative Medicine/Tissue Engineering
    Biomedical Engineering/Biotechnology
  • Buy this book on publisher's site
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