© 2020

3D Bioprinting

Principles and Protocols

  • Jeremy M. Crook

Part of the Methods in Molecular Biology book series (MIMB, volume 2140)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Generic Themes in 3D Bioprinting

    1. Front Matter
      Pages 1-1
    2. Patrick Thayer, Hector Martinez, Erik Gatenholm
      Pages 3-18
    3. Eliza Goddard, Susan Dodds
      Pages 43-64
    4. Kelsey Willson, Dongxu Ke, Carlos Kengla, Anthony Atala, Sean V. Murphy
      Pages 65-92
    5. Hitendra Kumar, Keekyoung Kim
      Pages 93-108
  3. Protocols for 3D Bioprinting

    1. Front Matter
      Pages 109-109
    2. Cathal O’Connell, Junxiang Ren, Leon Pope, Yifan Li, Anushree Mohandas, Romane Blanchard et al.
      Pages 111-133
    3. Davit Hakobyan, Olivia Kerouredan, Murielle Remy, Nathalie Dusserre, Chantal Medina, Raphael Devillard et al.
      Pages 135-144
    4. Serena Duchi, Carmine Onofrillo, Cathal O’Connell, Gordon G. Wallace, Peter Choong, Claudia Di Bella
      Pages 145-157
    5. Yang Wu, Yahui Zhang, Yin Yu, Ibrahim T. Ozbolat
      Pages 171-181
    6. Manuela Estermann, Christoph Bisig, Dedy Septiadi, Alke Petri-Fink, Barbara Rothen-Rutishauser
      Pages 199-215
    7. Cristina Quílez, Gonzalo de Aranda Izuzquiza, Marta García, Verónica López, Andrés Montero, Leticia Valencia et al.
      Pages 217-228
    8. Catherine Ngan, Anita Quigley, Cathal O’Connell, Magdalena Kita, Justin Bourke, Gordon G. Wallace et al.
      Pages 229-242
    9. Ganokon Urkasemsin, Sasitorn Rungarunlert, João N. Ferreira
      Pages 243-249

About this book


This volume explores the latest developments and contributions to the field of 3D bioprinting, and discusses its use for quality R&D and translation. The chapters in this book are divided into two parts: Part one covers generic themes in bioprinting to introduce novice readers to the field, while also providing experts with new and helpful information. Part two discusses protocols used to prepare, characterize, and print a variety of biomaterials, cells, and tissues. These chapters also emphasize methods used for printing defined and humanized constructs suitable for human tissue modelling in research and applicable to clinical product development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, 3D Bioprinting: Methods and Protocols is a valuable resource for researchers and bioprinting laboratories/facilities interested in learning more about this rapidly evolving technology.   


Bioprinted 3D Tissues Customised Bioinks Printed Tissues Stereolithography Neural Tissue

Editors and affiliations

  • Jeremy M. Crook
    • 1
  1. 1.ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation CampusUniversity of WollongongSquires Way, Fairy MeadowAustralia

Bibliographic information

  • Book Title 3D Bioprinting
  • Book Subtitle Principles and Protocols
  • Editors Jeremy M. Crook
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0519-6
  • Softcover ISBN 978-1-0716-0522-6
  • eBook ISBN 978-1-0716-0520-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XII, 260
  • Number of Illustrations 4 b/w illustrations, 50 illustrations in colour
  • Topics Biotechnology
  • Buy this book on publisher's site
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