© 2020

Fasciola hepatica

Methods and Protocols

  • Martin Cancela
  • Gabriela Maggioli

Part of the Methods in Molecular Biology book series (MIMB, volume 2137)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Valeria Gayo, Martin Cancela, Daniel Acosta
    Pages 1-14
  3. M. T. Ruiz-Campillo, V. Molina-Hernández, J. Pérez, I. L. Pacheco, R. Pérez, A. Escamilla et al.
    Pages 15-25
  4. Eduardo de la Torre-Escudero, Mark W. Robinson
    Pages 27-36
  5. Alicia Galiano, Maria Teresa Minguez, Christian M. Sánchez, Antonio Marcilla
    Pages 37-50
  6. Gabriel Rinaldi, Nicolás Dell’Oca, Estela Castillo, José F. Tort
    Pages 67-92
  7. Robin J. Flynn, Mayowa Musah-Eroje
    Pages 107-115
  8. Lorena Guasconi, Marianela C. Serradell, Diana T. Masih, Laura S. Chiapello
    Pages 117-131
  9. Marianela C. Serradell, Lorena Guasconi, Laura Cervi, Laura S. Chiapello, Diana T. Masih
    Pages 133-148
  10. Gabriela Maggioli, Cecilia Salazar, Federico Fossa, Carlos Carmona
    Pages 205-212
  11. Luis I. Alvarez, María Martinez Valladares, Candela Canton, Carlos E. Lanusse, Laura Ceballos
    Pages 213-220
  12. Florencia Ferraro, Mauricio A. Cabrera, Guzmán I. Álvarez, Ileana Corvo
    Pages 221-231
  13. Martin Cancela, Gabriela Maggioli
    Pages C1-C1
  14. Back Matter
    Pages 233-235

About this book


This volume described basic and advanced protocols to study F. hepatica parasite biology. Chapters guide readers through protocols on different developmental stages of F. hepatica, characterize tegumental, secreted proteins, spatial and temporal gene expression, immunological effects of ESP on macrophages, eosinophil apoptosis, macrophages alternative activation, toll-like receptor interactions, obtain peritoneal and splenic dendritic cells of mice, and protocols to detect F. hepatica albendazole resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Fasciola hepatica: Methods and Protocols provides different topics in areas such a biochemistry, immunology, molecular biology, microscopy, vaccinology in order to extend the interest in this book to research community working with Fasciola hepatica and related trematodes.


transgenesis selenium-proteins novel adjuvant systems liver fluke vaccines

Editors and affiliations

  • Martin Cancela
    • 1
  • Gabriela Maggioli
    • 2
  1. 1.Laboratório de Biologia Molecular de CestódeosCentro de Biotecnologia Universidade Federal do Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  2. 2.Unidad de Biología Parasitaria, Instituto de Higiene Facultad de CienciasUniversidad de la RepúblicaMontevideoUruguay

Bibliographic information

  • Book Title Fasciola hepatica
  • Book Subtitle Methods and Protocols
  • Editors Martin Cancela
    Gabriela Maggioli
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0474-8
  • Softcover ISBN 978-1-0716-0477-9
  • eBook ISBN 978-1-0716-0475-5
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 235
  • Number of Illustrations 13 b/w illustrations, 22 illustrations in colour
  • Topics Immunology
    Infectious Diseases
  • Buy this book on publisher's site
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