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Lectin Purification and Analysis

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2132)

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Table of contents (60 protocols)

  1. Functional Analysis of Botulinum Hemagglutinin (HA)

    • Takuhiro Matsumura, Yukako Fujinaga
    Pages 191-200
  2. Purification and Functional Characterization of the Effects on Cell Signaling of Mytilectin: A Novel β-Trefoil Lectin from Marine Mussels

    • Yuki Fujii, S. M. Abe Kawsar, Imtiaj Hasan, Hideaki Fujita, Marco Gerdol, Yasuhiro Ozeki
    Pages 201-213
  3. F-Type Lectins: Structure, Function, and Evolution

    • Gerardo R. Vasta, Chiguang Feng
    Pages 225-239
  4. Purification and Biochemical Characterization of Selected F-Type Lectins

    • Chiguang Feng, Gerardo R. Vasta
    Pages 241-255
  5. LecA (PA-IL): A Galactose-Binding Lectin from Pseudomonas aeruginosa

    • Sakonwan Kuhaudomlarp, Emilie Gillon, Annabelle Varrot, Anne Imberty
    Pages 257-266
  6. Glycan Recognition and Application of P-Type Lectins

    • Kei Kiriyama, Kohji Itoh
    Pages 267-276
  7. Purification and Assays of Tachylectin-5

    • Shun-ichiro Kawabata, Toshio Shibata
    Pages 277-283
  8. Preparation of Soluble Malectin and Its Tetramer

    • Sheng-Ying Qin, Dan Hu, Kazuo Yamamoto
    Pages 285-294
  9. Calnexin/Calreticulin and Assays Related to N-Glycoprotein Folding In Vitro

    • Yoshito Ihara, Midori Ikezaki, Maki Takatani, Yukishige Ito
    Pages 295-308
  10. Purification and Assays of Tachylectin-2

    • Shun-ichiro Kawabata, Toshio Shibata
    Pages 309-316
  11. Purification and Assays of Tachycitin

    • Shun-ichiro Kawabata, Toshio Shibata
    Pages 317-323
  12. ArtinM: Purification and Evaluation of Biological Activities

    • Thiago Aparecido da Silva, Patrícia Kellen Martins Oliveira-Brito, Sandra Maria de Oliveira Thomaz, Maria Cristina Roque-Barreira
    Pages 349-358
  13. Bacterial Expression of Rhamnose-Binding Lectin from Catfish Eggs

    • Shigeki Sugawara, Takeo Tatsuta, Masahiro Hosono
    Pages 359-367
  14. MIC4 from Toxoplasma gondii: A Lectin Acting as a Toll-Like Receptor Agonist

    • Flávia Costa Mendonça-Natividade, Rafael Ricci-Azevedo, Maria Cristina Roque-Barreira
    Pages 379-389
  15. Production and Characterization of MIC1: A Lectin from Toxoplasma gondii

    • Flávia Costa Mendonça-Natividade, Rafael Ricci-Azevedo, Sandra Maria de Oliveira Thomaz, Maria Cristina Roque-Barreira
    Pages 391-400

About this book

This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of the all the lectins—these chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems.



Editors and Affiliations

  • Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan

    Jun Hirabayashi

Bibliographic Information

  • Book Title: Lectin Purification and Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Jun Hirabayashi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0430-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0429-8Published: 18 April 2020

  • Softcover ISBN: 978-1-0716-0432-8Published: 18 April 2021

  • eBook ISBN: 978-1-0716-0430-4Published: 18 April 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 699

  • Number of Illustrations: 82 b/w illustrations, 110 illustrations in colour

  • Topics: Protein Science, Plant Sciences

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 239.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access