© 2020

Expression, Purification, and Structural Biology of Membrane Proteins

  • Camilo Perez
  • Timm Maier

Part of the Methods in Molecular Biology book series (MIMB, volume 2127)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Porsha L. R. Shaw, Kathryn A. Diederichs, Ashley Pitt, Sarah E. Rollauer, Susan K. Buchanan
    Pages 1-11
  3. Benjamin C. McIlwain, Ali A. Kermani
    Pages 13-27
  4. Jacques Boivineau, Matthias Haffke, Veli-Pekka Jaakola
    Pages 63-80
  5. Uwe Bodensohn, Christoph Ruland, Roman Ladig, Enrico Schleiff
    Pages 81-92
  6. Tamara Miljus, David A. Sykes, Clare R. Harwood, Ziva Vuckovic, Dmitry B. Veprintsev
    Pages 105-127
  7. Elisabeth Graeber, Volodymyr M. Korkhov
    Pages 129-137
  8. Benedikt T. Kuhn, Iwan Zimmermann, Pascal Egloff, Lea M. Hürlimann, Cedric A. J. Hutter, Christian Miscenic et al.
    Pages 151-165 Open Access
  9. Janine Denise Brunner, Stephan Schenck
    Pages 167-184
  10. Philipp A. M. Schmidpeter, Nattakan Sukomon, Crina M. Nimigean
    Pages 191-205
  11. Magnus Bloch, Mònica Santiveri, Nicholas M. I. Taylor
    Pages 227-244
  12. Valeria Kalienkova, Carolina Alvadia, Vanessa Clerico Mosina, Cristina Paulino
    Pages 245-273

About this book


This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins.

Chapter 11 is available open access under a CC-BY 4.0 license via


Membrane protein complexes Purification protocols Nanobodies Molecular mechanisms Expression yields

Editors and affiliations

  • Camilo Perez
    • 1
  • Timm Maier
    • 2
  1. 1.BiozentrumUniversity of BaselBaselSwitzerland
  2. 2.BiozentrumUniversity of BaselBaselSwitzerland

Bibliographic information

  • Book Title Expression, Purification, and Structural Biology of Membrane Proteins
  • Editors Camilo Perez
    Timm Maier
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0372-7
  • Softcover ISBN 978-1-0716-0375-8
  • eBook ISBN 978-1-0716-0373-4
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 423
  • Number of Illustrations 11 b/w illustrations, 78 illustrations in colour
  • Topics Protein Science
    Membrane Biology
  • Buy this book on publisher's site
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