© 2020

Innate Lymphoid Cells

Methods and Protocols

  • Shoba Amarnath

Part of the Methods in Molecular Biology book series (MIMB, volume 2121)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Matthew T. Stier, R. Stokes Peebles Jr
    Pages 7-22
  3. Gianluca Scarno, Giuseppe Pietropaolo, Chiara Di Censo, Giovanna Peruzzi, Giuseppe Sciumè
    Pages 59-70
  4. Brinda Bhatt, Huabin Zhu, Nikhil Patel, Nagendra Singh
    Pages 93-98
  5. Lewis J. Entwistle, Franz Puttur, Lisa G. Gregory, Clare M. Lloyd
    Pages 99-114
  6. Jonathan Silver, Alison A. Humbles, Yoichiro Ohne
    Pages 115-127
  7. Olivia Jane James, Maud Vandereyken, Mahima Swamy
    Pages 141-152
  8. Grace Mallett, Warren Patterson, Megan Payne, Shoba Amarnath
    Pages 153-164
  9. Christopher J. Stewart, Mary K. Estes, Sasirekha Ramani
    Pages 185-198
  10. Mélanie Lambert, Caroline Leijonhufvud, Filip Segerberg, J. Joseph Melenhorst, Mattias Carlsten
    Pages 213-239
  11. Back Matter
    Pages 241-242

About this book


This volume explores the latest techniques used to characterize murine and human Innate Lymphoid Cells (ILC) subsets. This book also aims to discuss useful phenotypical and functional assays that may be relevant for studying ILC function in mice and humans. Chapters cover topics such as methods to study microbiome and enteroid cultures; characterizing ILC precursors; and cellular engineering of ILCs for therapeutic use. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Innate Lymphoid Cells: Methods and Protocols is a valuable research tool for anyone interested in ILC biology, and those interested in fundamental immunology, cellular engineering, cell signaling, and clinician scientists. 


Developmental pathways Phenotype Characterization Parasitic Helminths Allergic Inflammation CD4+ and CD4- lymphoid tissue inducer cells

Editors and affiliations

  • Shoba Amarnath
    • 1
  1. 1.Laboratory of T cell Regulation, Institute of Cellular MedicineNewcastle UniversityNewcastle Upon TyneUK

Bibliographic information

  • Book Title Innate Lymphoid Cells
  • Book Subtitle Methods and Protocols
  • Editors Shoba Amarnath
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0337-6
  • Softcover ISBN 978-1-0716-0340-6
  • eBook ISBN 978-1-0716-0338-3
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIII, 242
  • Number of Illustrations 9 b/w illustrations, 39 illustrations in colour
  • Topics Cancer Research
    Cell Biology
  • Buy this book on publisher's site
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