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© 2020

RNA Interference and CRISPR Technologies

Technical Advances and New Therapeutic Opportunities

  • Mouldy Sioud
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2115)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Ivan V. Chernikov, Mariya I. Meschaninova, Elena L. Chernolovskaya
    Pages 57-77
  3. Seung Koo Lee, Benedict Law, Ching-Hsuan Tung
    Pages 79-90
  4. Shiva Kheiriabad, Maryam Ghaffari, Jafar Ezzati Nazhad Dolatabadi, Michael R. Hamblin
    Pages 91-106
  5. Lijia Yu, Duanwei Liang, Nannan Jing, Changmai Chen, Xinjing Tang
    Pages 133-161
  6. Fiona T. van den Berg, Abdullah Ely, Patrick Arbuthnot
    Pages 185-197
  7. Jaroslav Juracek, Ondrej Slaby
    Pages 221-247
  8. Stein Sæbøe-Larssen, Mouldy Sioud
    Pages 249-258
  9. Nicholas Paul Casey, Jon Amund Kyte, Hiroshi Fujiwara
    Pages 327-349
  10. Kasidet Hiranniramol, Yuhao Chen, Xiaowei Wang
    Pages 351-364

About this book

Introduction

This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine. 

Keywords

shRNAs mRNA sequence CRISPR Cas9 T cell therapy

Editors and affiliations

  • Mouldy Sioud
    • 1
  1. 1.Department of Cancer Immunology, Institute for Cancer ResearchOslo University Hospital-RadiumhospitaletOsloNorway

Bibliographic information

  • Book Title RNA Interference and CRISPR Technologies
  • Book Subtitle Technical Advances and New Therapeutic Opportunities
  • Editors Mouldy Sioud
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI https://doi.org/10.1007/978-1-0716-0290-4
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0289-8
  • Softcover ISBN 978-1-0716-0292-8
  • eBook ISBN 978-1-0716-0290-4
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XVI, 486
  • Number of Illustrations 22 b/w illustrations, 48 illustrations in colour
  • Topics Gene Expression
    Genetics and Genomics
  • Buy this book on publisher's site
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