RNA Interference and CRISPR Technologies

Technical Advances and New Therapeutic Opportunities

  • Mouldy Sioud

Part of the Methods in Molecular Biology book series (MIMB, volume 2115)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Ivan V. Chernikov, Mariya I. Meschaninova, Elena L. Chernolovskaya
    Pages 57-77
  3. Seung Koo Lee, Benedict Law, Ching-Hsuan Tung
    Pages 79-90
  4. Shiva Kheiriabad, Maryam Ghaffari, Jafar Ezzati Nazhad Dolatabadi, Michael R. Hamblin
    Pages 91-106
  5. Lijia Yu, Duanwei Liang, Nannan Jing, Changmai Chen, Xinjing Tang
    Pages 133-161
  6. Fiona T. van den Berg, Abdullah Ely, Patrick Arbuthnot
    Pages 185-197
  7. Jaroslav Juracek, Ondrej Slaby
    Pages 221-247
  8. Stein Sæbøe-Larssen, Mouldy Sioud
    Pages 249-258
  9. Nicholas Paul Casey, Jon Amund Kyte, Hiroshi Fujiwara
    Pages 327-349
  10. Kasidet Hiranniramol, Yuhao Chen, Xiaowei Wang
    Pages 351-364
  11. Nirakar Sahoo, Victoria Cuello, Shreya Udawant, Carl Litif, Julie A. Mustard, Megan Keniry
    Pages 365-383
  12. Sergey V. Prykhozhij, Anna Cordeiro-Santanach, Lucia Caceres, Jason N. Berman
    Pages 385-405
  13. Xingying Zhang, Chen Cheng, Wen Sun, Haoyi Wang
    Pages 419-433
  14. Kanut Laoharawee, Matthew J. Johnson, Branden S. Moriarity
    Pages 435-444
  15. Baoyan Bai, June Helen Myklebust, Sébastien Wälchli
    Pages 445-454
  16. Perihan Mir, Malte Ritter, Karl Welte, Julia Skokowa, Maksim Klimiankou
    Pages 455-469
  17. Benjamin Dannenmann, Masoud Nasri, Karl Welte, Julia Skokowa
    Pages 471-483
  18. Back Matter
    Pages 485-486

About this book


This volume explores the uses of RNAi and CRISPR interferences as a general method for inhibiting gene expression, with focus on their biological functions, design, chemical modifications, delivery, and preclinical/clinical applications. In addition to relevant backgrounds, the chapters in this book discuss simple and accurate protocols dealing with the latest advances in RNAi and CRISPR applications and look at how these methods differ from one another. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, RNA Interference and CRISPR Technologies: Technical Advances and New Therapeutic Opportunities is a valuable resource for any scientist, teachers, graduate student, postdoc, and clinician interested in this field. This book also benefits anyone in research and development in biotech and pharmaceutical companies who want to understand more about these technologies, and their applications in biology and medicine. 


shRNAs mRNA sequence CRISPR Cas9 T cell therapy

Editors and affiliations

  • Mouldy Sioud
    • 1
  1. 1.Department of Cancer Immunology, Institute for Cancer ResearchOslo University Hospital-RadiumhospitaletOsloNorway

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0289-8
  • Online ISBN 978-1-0716-0290-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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