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Principles and Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing

  • Hirak Ranjan Dash
  • Pankaj Shrivastava
  • Surajit Das
Book

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xix
  2. Overview of Forensic DNA Analysis

    1. Front Matter
      Pages 1-1
    2. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 3-11
    3. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 13-20
    4. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 21-27
  3. Manual Extraction of DNA from Biological Samples

    1. Front Matter
      Pages 29-29
    2. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 31-37
    3. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 39-45
    4. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 47-52
    5. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 53-59
    6. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 61-67
    7. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 69-76
    8. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 77-84
  4. Automatic and Semi-automatic Extraction of DNA from Biological Samples

    1. Front Matter
      Pages 85-85
    2. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 87-96
    3. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 97-101
    4. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 103-108
    5. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 109-115
  5. Qualitative and Quantitative Assessment of Extracted DNA

    1. Front Matter
      Pages 117-117
    2. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 119-125
    3. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 127-132
    4. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 133-147
    5. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 149-154
  6. Amplification of Various STR Markers Using Multiplex PCR

    1. Front Matter
      Pages 155-155
    2. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 157-163
    3. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 165-171
    4. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 173-178
    5. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 179-184
  7. Genotyping and Analysis of Results

    1. Front Matter
      Pages 185-185
    2. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 187-212
    3. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 213-237
    4. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 239-251
  8. Case Studies

    1. Front Matter
      Pages 253-253
    2. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 255-259
    3. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 261-265
    4. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 267-273
    5. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 275-280
  9. Advanced Technologies for Forensic DNA Analysis

    1. Front Matter
      Pages 281-281
    2. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 283-292
    3. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 293-303
    4. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 305-311
    5. Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
      Pages 313-316
  10. Back Matter
    Pages 317-344

About this book

Introduction

The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples.

It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples.

Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis.

Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications. 

      

Keywords

DNA Fingerprinting Protocols STR markers DNA Genotyping NGS Mitochondrial DNA analysis

Authors and affiliations

  • Hirak Ranjan Dash
    • 1
  • Pankaj Shrivastava
    • 2
  • Surajit Das
    • 3
  1. 1.DNA Fingerprinting UnitState Forensic Science LaboratorySagarIndia
  2. 2.DNA Fingerprinting UnitState Forensic Science LaboratorySagarIndia
  3. 3.Department of Life ScienceNational Institute of TechnologyRourkelaIndia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-0716-0274-4
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0273-7
  • Online ISBN 978-1-0716-0274-4
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site
Industry Sectors
Biomedicine
Pathology