T-Cell Receptor Signaling

Methods and Protocols

  • Chaohong Liu

Part of the Methods in Molecular Biology book series (MIMB, volume 2111)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Kaitlin C. O’Boyle, Takuya Ohtani, Sasikanth Manne, Bertram Bengsch, Sarah E. Henrickson, E. John Wherry et al.
    Pages 1-20
  3. Xian Jia, Xiaojuan Zhou, Haiping Zheng, Shan Jiang, Jiannan Weng, Lei Huang et al.
    Pages 21-33
  4. Wanjing Shang, Fei Wang, Qi Zhu, Liangyu Wang, Haopeng Wang
    Pages 59-70
  5. Jaclyn R. Espinosa, Joshua D. Wheaton, Maria Ciofani
    Pages 79-89
  6. Wenyong Yang, Xueying Chen, Hongbo Hu
    Pages 91-99
  7. Yang Zhang, Xuehui Long, Xiaoming Wang
    Pages 115-126
  8. Makoto Ando, Mari Ikeda, Akihiko Yoshimura, Taisuke Kondo
    Pages 127-139
  9. Yi-Geng Pang, Chien-Chung Chang
    Pages 141-151
  10. Kendra A. Libby, Xiaolei Su
    Pages 153-160
  11. Tyng-An Zhou, Chia-Lin Hsu, Ivan Lilyanov Dzhagalov
    Pages 205-219
  12. Natania S. Field, Claire E. O’Leary, Joseph M. Dybas, Hua Ding, Paula M. Oliver
    Pages 239-256
  13. Xuyong Chen, John William Sherman, Ruoning Wang
    Pages 257-265
  14. Moutuaata M. Moutuou, Simon-David Gauthier, Nicolas Chen, Dominique Leboeuf, Martin Guimond
    Pages 267-283
  15. Yu Liu, Wei Wang, Xiongwen Wu, Xiufang Weng
    Pages 285-293
  16. Back Matter
    Pages 295-296

About this book


This volume provides current and new advanced methods and protocols to study T cells. Chapters guide readers through T cell diversity using mass cytometry, analyzing T cells from single cell level, CRISPR/Cas9 techniques to study the T cell activation, techniques to study subsets of Tcell’s, procedures to study artificial antigen presentosomes for T cell activation, techniques to study the T cell development, two-photon microscopy, and MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, T-Cell Receptor Signaling: Methods and Protocols aims to provide a wide range of approaches and be an invaluable resource for present and future generations of T cell researchers.


cell metabolism miRNA expression two-photon microscopy Treg re-sorting immunosorbent spot assays

Editors and affiliations

  • Chaohong Liu
    • 1
  1. 1.Department of Pathogen BiologyHuazhong University of Science and TechnologyWuhanChina

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0265-2
  • Online ISBN 978-1-0716-0266-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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