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Immune Mediators in Cancer

Methods and Protocols

  • Ivana Vancurova
  • Yan Zhu

Part of the Methods in Molecular Biology book series (MIMB, volume 2108)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Detection of Immune Mediators in Cancer Cells and Tissues

    1. Front Matter
      Pages 1-1
    2. Weiqiang Chen, Guoqiang Bao, Lin Zhao, Haichao Wang
      Pages 15-28
    3. Zhuang Ye, Ling Zhao, Qi Gao, Yanfang Jiang, Zhenyu Jiang, Cong-Qiu Chu
      Pages 29-42
    4. Helena Kupcova Skalnikova, Katerina Vodickova Kepkova, Petr Vodicka
      Pages 65-88
  3. Cytokine Bioassays

    1. Front Matter
      Pages 99-99
    2. Bijaya Gaire, Mohammad M. Uddin, Yue Zou, Ivana Vancurova
      Pages 101-106
    3. Mohammad M. Uddin, Bijaya Gaire, Betsy Deza, Ivana Vancurova
      Pages 107-115
    4. Mohammad M. Uddin, Bijaya Gaire, Ivana Vancurova
      Pages 117-124
    5. Alejandra M. Martinez, Ahryun Kim, Wan Seok Yang
      Pages 125-130
    6. Sheyda Najafi, Ehab M. Abo-Ali, Vikas V. Dukhande
      Pages 131-146
    7. Wenjun Wu, Zhihui Xiao, Deon Buritis, Vladimir Poltoratsky
      Pages 167-179
    8. Shao Tao, Pingfang Song, Xiaowei Zhang, Lingshu Zhang, Cong-Qiu Chu
      Pages 181-196
    9. Pritpal Kaur, Shreya Nagar, Madhura Bhagwat, Mohammad M. Uddin, Yan Zhu, Ales Vancura
      Pages 197-207
  4. Expression and Regulation of Immune Mediators in Cancer Cells

    1. Front Matter
      Pages 209-209
    2. Ruizhi Wang, Md. Faruk Hossain, Jovan Mirkovic, Samuel Sabzanov, Matteo Ruggiu
      Pages 241-258
    3. Julian J. Freen-van Heeren, Benoit P. Nicolet, Monika C. Wolkers
      Pages 259-271
    4. Kiersten P. Carter, Jeffrey E. Segall, Dianne Cox
      Pages 273-279
    5. Maren Hülsemann, Polina V. Verkhusha, Peng Guo, Veronika Miskolci, Dianne Cox, Louis Hodgson
      Pages 281-293
  5. Bioinformatics and Genome Editing Approaches

  6. Back Matter
    Pages 371-373

About this book

Introduction

This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity in cancer cells and tissues. Beginning with a section on the detection of immune mediators in samples, the volume continues with sections covering cytokine bioassays, the expression and regulation of immune mediators in cancer cells, and methods to navigate the enormous datasets created by modern DNA and RNA sequencing and proteomic technology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and wide-ranging, Immune Mediators in Cancer: Methods and Protocols serves as a valuable resource for biochemists, molecular biologists, cancer biologists, and immunologists, as well as for physician-scientists working in the field of immunology and cancer research.

Keywords

Tumor cells Cytokines Tumor microenvironment Immune checkpoints Bioinformatics Immune cells Cancer cells and tissues

Editors and affiliations

  • Ivana Vancurova
    • 1
  • Yan Zhu
    • 2
  1. 1.Department of Biological SciencesSt. John’s UniversityQueensUSA
  2. 2.Department of Biological SciencesSt. John’s UniversityQueensUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-0716-0247-8
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0246-1
  • Online ISBN 978-1-0716-0247-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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