Table of contents
About this book
This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity in cancer cells and tissues. Beginning with a section on the detection of immune mediators in samples, the volume continues with sections covering cytokine bioassays, the expression and regulation of immune mediators in cancer cells, and methods to navigate the enormous datasets created by modern DNA and RNA sequencing and proteomic technology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and wide-ranging, Immune Mediators in Cancer: Methods and Protocols serves as a valuable resource for biochemists, molecular biologists, cancer biologists, and immunologists, as well as for physician-scientists working in the field of immunology and cancer research.
Tumor cells Cytokines Tumor microenvironment Immune checkpoints Bioinformatics Immune cells Cancer cells and tissues
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-0716-0247-8
- Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
- Publisher Name Humana, New York, NY
- eBook Packages Springer Protocols
- Print ISBN 978-1-0716-0246-1
- Online ISBN 978-1-0716-0247-8
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
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