© 2020

Cytoskeleton Dynamics

Methods and Protocols

  • Helder Maiato

Part of the Methods in Molecular Biology book series (MIMB, volume 2101)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Jeffrey O. Spector, Annapurna Vemu, Antonina Roll-Mecak
    Pages 39-51
  3. Kim J. A. Vendel, Celine Alkemade, Nemo Andrea, Gijsje H. Koenderink, Marileen Dogterom
    Pages 53-75
  4. Virginie Stoppin-Mellet, Nassiba Bagdadi, Yasmina Saoudi, Isabelle Arnal
    Pages 77-91
  5. Charlène Cornilleau, Arnaud Chastanet, Cyrille Billaudeau, Rut Carballido-López
    Pages 123-133
  6. Cyrille Billaudeau, Arnaud Chastanet, Rut Carballido-López
    Pages 135-145
  7. Catarina Peneda, Carla A. M. Lopes, Mónica Bettencourt-Dias
    Pages 147-162
  8. Artur Ezquerra, Ricardo Viais, Jens Lüders
    Pages 163-178
  9. Roslin J. Thoppil, Anna A. W. M. Sanders, Irina Kaverina
    Pages 179-189
  10. J. Richard McIntosh, Eileen O’Toole, Cynthia Page, Garry Morgan
    Pages 191-209
  11. Jeffrey van Haren, Lauren S. Adachi, Torsten Wittmann
    Pages 211-234
  12. Ana C. Almeida, Danica Drpic, Naoyuki Okada, Joana Bravo, Marta Madureira, Helder Maiato
    Pages 247-266
  13. Fung Yi Chan, Ana Marta Silva, Ana Xavier Carvalho
    Pages 297-325

About this book


This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers. 

The chapter “Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes” is available open access under a Creative Commons Attribution 4.0 International License via


microtubules Golgi-mediated microtubule organization protein purification in vitro reconstitution cytoskeleton properties nuclear migration

Editors and affiliations

  • Helder Maiato
    • 1
  1. 1.i3S University of PortoPortoPortugal

Bibliographic information

  • Book Title Cytoskeleton Dynamics
  • Book Subtitle Methods and Protocols
  • Editors Helder Maiato
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0218-8
  • Softcover ISBN 978-1-0716-0221-8
  • eBook ISBN 978-1-0716-0219-5
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 390
  • Number of Illustrations 12 b/w illustrations, 72 illustrations in colour
  • Topics Cell Biology
    Protein Science
  • Buy this book on publisher's site
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