© 2020

MAIT Cells

Methods and Protocols

  • Helen Kaipe
  • Isabelle Magalhaes

Part of the Methods in Molecular Biology book series (MIMB, volume 2098)

Table of contents

  1. Front Matter
    Pages i-xii
  2. MAIT Cells in Human Tissues and Blood

    1. Front Matter
      Pages 1-1
    2. Isabelle Magalhaes, Martin Solders, Helen Kaipe
      Pages 3-21
    3. Daniela Di Blasi, Alessandro Vacchini, Gennaro De Libero, Lucia Mori
      Pages 23-38
    4. William Rodin, Patrik Sundström, Marianne Quiding Järbrink
      Pages 39-53
    5. Benedikt Strunz, Christine L. Zimmer, Jonna Bister, Martin A. Ivarsson, Niklas K. Björkström
      Pages 71-82
    6. Anna Gibbs, Michal J. Sobkowiak, Johan K. Sandberg, Annelie Tjernlund
      Pages 83-94
  3. Functional Analysis of MAIT Cells

    1. Front Matter
      Pages 95-95
    2. Joachim P. Hagel, Lucy C. Garner, Matthew Bilton, Hema Mehta, Tianqi Leng, Carl-Philipp Hackstein et al.
      Pages 97-124
    3. Mariolina Salio, Vincenzo Cerundolo
      Pages 125-139
    4. Liyen Loh, Marios Koutsakos, Katherine Kedzierska, Timothy S. C. Hinks
      Pages 141-147 Open Access
    5. Wan Rong Sia, Caroline Boulouis, Muhammad Yaaseen Gulam, Andrea Lay Hoon Kwa, Johan K. Sandberg, Edwin Leeansyah
      Pages 149-165
    6. Susanne Jahreis, Sarah Boettcher, Marie von Lilienfeld-Toal
      Pages 167-177
    7. Farhat Parween, Hongwei H. Zhang, Joshua M. Farber
      Pages 179-188
  4. Monitoring and In Vitro Amplification of MAIT Cells

    1. Front Matter
      Pages 189-189
    2. Nicole A. Ladd, Cara A. Froyd, Erin J. Adams
      Pages 191-207
    3. Isabel Bernal, Marco van Ham, Lothar Jänsch
      Pages 209-218
    4. Meghan Cansler, Megan Null, Erin Meermeier, Gwendolyn Swarbrick, David Lewinsohn, Melanie Harriff
      Pages 219-235

About this book


This volume focuses on various methods used by researchers to study mucosal-associated invariant T (MAIT) cells in the aspects described below. The chapters are organized into Four Parts: part One looks at the function and importance of MAIT cells in health and disease. It covers methods to isolate and characterize MAIT cells from human tissues including liver, colon tumors, placenta and decidua, and endometrium. Part Two discusses MR1 independent stimulation, and looks at the activation of MAIT cells by different stimulatory agents. Part Three describes various methods to develop tools to analyze, monitor, and generate MAIT cells in vitro, including the production of MR1-tetramers and how they can be loaded with bacterial antigens and used for the detection of MAIT cells. The last part covers the use of murine models to study MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, MAIT Cells: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.

Chapters 9 and 17 are available open access under a Creative Commons Attribution 4.0 International License via


microscopy flow cytometry mouse models MAIT heterogeneity MR1 expressing cells

Editors and affiliations

  • Helen Kaipe
    • 1
  • Isabelle Magalhaes
    • 2
  1. 1.Department of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenClinical Immunology and Transfusion Medicine, Karolinska University HospitalStockholmSweden
  2. 2.Department of Oncology-PathologyKarolinska InstitutetStockholmSweden

Bibliographic information

  • Book Title MAIT Cells
  • Book Subtitle Methods and Protocols
  • Editors Helen Kaipe
    Isabelle Magalhaes
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0206-5
  • Softcover ISBN 978-1-0716-0209-6
  • eBook ISBN 978-1-0716-0207-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XII, 310
  • Number of Illustrations 13 b/w illustrations, 41 illustrations in colour
  • Topics Immunology
    Cell Biology
  • Buy this book on publisher's site
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