Cell Reprogramming for Immunotherapy

Methods and Protocols

  • Samuel G. Katz
  • Peter M. Rabinovich

Part of the Methods in Molecular Biology book series (MIMB, volume 2097)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Reprogramming T and NK Cells

    1. Front Matter
      Pages 1-1
    2. Samuel G. Katz, Peter M. Rabinovich
      Pages 3-44
    3. Reginald Tran, Wilbur A. Lam
      Pages 55-65
    4. Roddy S. O’Connor, Michael C. Milone
      Pages 83-90
    5. Noriko Shimasaki, Dario Campana
      Pages 91-105
    6. Nathan J. Schloemer, Alex M. Abel, Monica S. Thakar, Subramaniam Malarkannan
      Pages 115-123
    7. Carolina I. Domaica, Jessica M. Sierra, Norberto W. Zwirner, Mercedes B. Fuertes
      Pages 125-136
  3. Reprogramming Diverse Immunocytes

    1. Front Matter
      Pages 137-137
    2. Jialing Zhang, Stephan S. Späth, Sherman M. Weissman, Samuel G. Katz
      Pages 139-171
    3. Douglas Hanlon, Olga Sobolev, Patrick Han, Alessandra Ventura, Aaron Vassall, Nour Kibbi et al.
      Pages 173-195
    4. Qinqin Cheng, Xiaojing Shi, Yong Zhang
      Pages 197-209
    5. Ketki Bhise, Samaresh Sau, Rami Alzhrani, Arun K. Iyer
      Pages 211-221
  4. Monitoring Reprogrammed Immune Cells In Vivo

    1. Front Matter
      Pages 265-265
    2. Yuanbin Song, Rana Gbyli, Xiaoying Fu, Stephanie Halene
      Pages 273-289
  5. Clinical Design for Cell Therapy

    1. Front Matter
      Pages 291-291
    2. Agnieszka Jozwik, Alan Dunlop, Katy Sanchez, Reuben Benjamin
      Pages 293-308
    3. Bryden Considine, Michael E. Hurwitz
      Pages 309-327
    4. Alexey Bersenev, Andrew Fesnak
      Pages 329-339
  6. Back Matter
    Pages 341-343

About this book


This volume details key protocols for developing strategies in immunotherapy.  Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Immunocytes Immune cells NK cells CAR signaling lentivirus oncolytic viruses tumor infiltrating lymphocytes

Editors and affiliations

  • Samuel G. Katz
    • 1
  • Peter M. Rabinovich
    • 2
  1. 1.Department of PathologyYale School of MedicineNew HavenUSA
  2. 2.Department of PathologyYale School of MedicineNew HavenUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0202-7
  • Online ISBN 978-1-0716-0203-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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